Peng Qianze, Sun Shu'e, Ma Jiejia, Chen Silu, Gao Liming, Du Xiaohua, Liu Xian, Zhu Feiying, Peng Weiye, Liu Yong, Su Pin, Cernava Tomislav, Zhang Deyong
School of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China.
State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Environ Microbiome. 2025 Jul 11;20(1):86. doi: 10.1186/s40793-025-00748-9.
The plant microbiome can support plant health and fitness in the face of biotic and abiotic stress. Research has mostly focused on plant growth in natural and agricultural soils. However, as urban areas continue to expand and soils change in the Anthropocene, microbiome assembly during development of plants grown in urban area soil remains largely elusive. Here, we examined the effect of developmental stages on the phyllosphere and rhizosphere microbiomes of rice grown in soil from an urban area during the vegetative growth stages.
We found that the microbial alpha and beta diversity, networks, and functions of the phyllosphere and rhizosphere microbiomes significantly differed among rice seedling, tillering, and elongation stages. Notably, we observed that bacteria assigned to potential animal parasites or symbionts not only exhibited significantly higher relative abundances in the phyllosphere compared to the rhizosphere but are also influenced by the developmental stages. Plants grown in the urban area soil had a higher relative abundance of Bacteroidales and enriched bacteria assigned to potential animal parasites or symbionts in the phyllosphere in contrast to plants grown in field. Some of these bacteria were shown to significantly influence the assembly of the phyllosphere microbiome and to prevalently engage in negative interactions with other microbes.
Our study provides new insights into developmental stage-resolved microbiome assembly of plants grown in urban areas. The insights could help in the development of strategies for promoting 'One Health' by highlighting the role of plants as alternative host for bacterial groups that are prevalently associated with animals.
植物微生物群能够在面对生物和非生物胁迫时支持植物的健康和适应性。研究大多集中在自然土壤和农业土壤中的植物生长。然而，随着城市地区不断扩张以及土壤在人类世发生变化，城市地区土壤中生长的植物在发育过程中的微生物群落组装情况仍 largely 难以捉摸。在此，我们研究了发育阶段对营养生长阶段在城市地区土壤中种植的水稻叶际和根际微生物群的影响。
我们发现，水稻幼苗期、分蘖期和拔节期的叶际和根际微生物群的微生物α和β多样性、网络及功能存在显著差异。值得注意的是，我们观察到被归类为潜在动物寄生虫或共生体的细菌不仅在叶际中的相对丰度显著高于根际，而且还受发育阶段影响。与在田间种植的植物相比，在城市地区土壤中生长的植物叶际中拟杆菌目相对丰度更高，且富集了被归类为潜在动物寄生虫或共生体的细菌。其中一些细菌被证明对叶际微生物群的组装有显著影响，并普遍与其他微生物发生负相互作用。
我们的研究为城市地区生长植物的发育阶段解析微生物群落组装提供了新见解。这些见解有助于通过强调植物作为与动物普遍相关的细菌群体的替代宿主的作用来制定促进“同一健康”的策略。
