Flament-Simon Saskia-Camille, Nicolas-Chanoine Marie-Hélène, García Vanesa, Duprilot Marion, Mayer Noémie, Alonso María Pilar, García-Meniño Isidro, Blanco Jesús E, Blanco Miguel, Blanco Jorge
Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, Spain.
Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain.
Antibiotics (Basel). 2020 Apr 4;9(4):161. doi: 10.3390/antibiotics9040161.
is the main pathogen responsible for extraintestinal infections. A total of 196 clinical consecutively isolated during 2016 in Spain (100 from Lucus Augusti hospital in Lugo) and France (96 from Beaujon hospital in Clichy) were characterized. Phylogroups, clonotypes, sequence types (STs), O:H serotypes, virulence factor (VF)-encoding genes and antibiotic resistance were determined. Approximately 10% of the infections were caused by ST131 isolates in both hospitals and approximately 60% of these infections were caused by isolates belonging to only 10 STs (ST10, ST12, ST58, ST69, ST73, ST88, ST95, ST127, ST131, ST141). ST88 isolates were frequent, especially in Spain, while ST141 isolates significantly predominated in France. The 23 ST131 isolates displayed four clonotypes: CH40-30, CH40-41, CH40-22 and CH40-298. Only 13 (6.6%) isolates were carriers of extended-spectrum beta-lactamase (ESBL) enzymes. However, 37.2% of the isolates were multidrug-resistant (MDR). Approximately 40% of the MDR isolates belonged to only four of the dominant clones (B2-CH40-30-ST131, B2-CH40-41-ST131, C-CH4-39-ST88 and D-CH35-27-ST69). Among the remaining MDR isolates, two isolates belonged to B2-CH14-64-ST1193, i.e., the new global emergent MDR clone. Moreover, a hybrid extraintestinal pathogenic (ExPEC)/enteroaggregative isolate belonging to the A-CH11-54-ST10 clone was identified.
是引起肠外感染的主要病原体。对2016年在西班牙（100株来自卢戈的卢库斯·奥古斯蒂医院）和法国（96株来自克利希的博若医院）连续分离出的196株临床菌株进行了特征分析。确定了菌群、克隆型、序列类型（STs）、O:H血清型、毒力因子（VF）编码基因和抗生素耐药性。两家医院约10%的感染由ST131分离株引起，其中约60%的感染由仅属于10种STs（ST10、ST12、ST58、ST69、ST73、ST88、ST95、ST127、ST131、ST141）的分离株引起。ST88分离株很常见，尤其是在西班牙，而ST141分离株在法国显著占主导地位。23株ST131分离株表现出四种克隆型：CH40 - 30、CH40 - 41、CH40 - 22和CH40 - 298。只有13株（6.6%）分离株是超广谱β-内酰胺酶（ESBL）酶的携带者。然而，37.2%的分离株是多重耐药（MDR）的。约40%的MDR分离株仅属于四个优势克隆（B2 - CH40 - 30 - ST131、B2 - CH40 - 41 - ST131、C - CH4 - 39 - ST88和D - CH35 - 27 - ST69）。在其余的MDR分离株中，有两株属于B2 - CH14 - 64 - ST1193，即新出现的全球MDR克隆。此外，还鉴定出一株属于A - CH11 - 54 - ST10克隆的混合型肠外致病性（ExPEC）/肠聚集性分离株。
