Silva Vanessa, Caniça Manuela, de la Rivière Rani, Barros Paulo, Cabral João Alexandre, Poeta Patrícia, Igrejas Gilberto
LAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade Nova de Lisboa, 1099-085 Caparica, Portugal.
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Vet Sci. 2025 Apr 1;12(4):322. doi: 10.3390/vetsci12040322.
Bats are increasingly recognized as reservoirs for antimicrobial-resistant bacteria, playing a potential role in the dissemination of resistance genes across species and regions. In this study, 105 bats from 19 species in Portugal were sampled to investigate the presence, antimicrobial resistance, and genetic characteristics of and isolates. Thirteen and were recovered. Antimicrobial susceptibility testing revealed multidrug resistance in three isolates, with carrying (C), (A), and C genes, and harboring B, (K), and . Notably, A was detected in , highlighting its plasmid-associated potential for horizontal gene transfer to more pathogenic bacteria. Heavy metal resistance genes (B and D) were also identified, suggesting the role of environmental factors in co-selecting antimicrobial resistance. Molecular typing revealed the strain as ST297, a clone associated with both healthy humans and invasive infections. These findings emphasize the need for monitoring bats as reservoirs of resistance determinants, particularly in the context of zoonotic and environmental health. The presence of mobile genetic elements and plasmids further underscores the potential for the dissemination of resistance. This study reinforces the importance of adopting a One Health approach to mitigate the risks associated with antimicrobial resistance.
蝙蝠越来越被认为是抗微生物药物耐药细菌的宿主，在耐药基因跨物种和跨区域传播中发挥着潜在作用。在本研究中，对葡萄牙19个物种的105只蝙蝠进行了采样，以调查[具体细菌名称]分离株的存在情况、抗微生物药物耐药性及遗传特征。共分离出13株[具体细菌名称]。抗微生物药物敏感性试验显示，3株分离株具有多重耐药性，其中一株携带[具体基因名称1]（C）、[具体基因名称2]（A）和C基因，另一株携带B、[具体基因名称3]（K）和[具体基因名称4]。值得注意的是，在[具体细菌名称]中检测到了A，突出了其通过质粒进行水平基因转移至更多致病性细菌的潜力。还鉴定出了重金属抗性基因（B和D），表明环境因素在共同选择抗微生物药物耐药性方面的作用。分子分型显示[具体细菌名称]菌株为ST297，这是一种与健康人和侵袭性感染均相关的克隆。这些发现强调了将蝙蝠作为耐药决定因素宿主进行监测的必要性，特别是在人畜共患病和环境卫生背景下。移动遗传元件和质粒的存在进一步强调了耐药性传播的可能性。本研究强化了采用“同一健康”方法来降低与抗微生物药物耐药性相关风险的重要性。
