Sun Jiazheng, Li Shiling, Shao Xijun, Fang Mingxuan, Zhang Heng, Zhu Zhiheng, Sun Xia
School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China.
Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, Zibo 255049, China.
Sensors (Basel). 2025 Apr 20;25(8):2602. doi: 10.3390/s25082602.
Procymidone (PCM) is an effective, low-toxicity fungicide commonly used to control plant diseases in grains, vegetables, and fruits. Its usage has significantly increased in recent years, resulting in higher residues in vegetables. This study developed a sensitive and rapid immunoassay method utilizing a gold- and fluorescence-labeled monoclonal antibody (mAb) for detecting PCM residues in vegetable samples. Under optimal conditions, the fluorescent microsphere-labeled monoclonal antibody immunochromatographic strips achieved a limit of detection (LOD) of 1.67 ng/mL, with a visual LOD of 50 ng/mL. Intra-batch accuracy ranged from 94.98% to 103.82%, with a coefficient of variation (CV) of 1.97% to 8.26%. Inter-batch accuracy ranged from 96.16% to 102.51%, with a CV of 4.62% to 8.91%. The visual detection range of the gold nanoparticle-labeled monoclonal antibody immunochromatographic strips was 50 to 200 ng/g. The method demonstrated excellent performance in actual vegetable samples, confirming its applicability across various matrices. This dual-method approach enables rapid screening of negative samples with gold test strips, followed by accurate quantitative analysis of positive samples using fluorescent test strips, thereby enhancing efficiency and addressing diverse detection needs. Consequently, this method holds significant market potential for practical applications.
腐霉利（PCM）是一种高效、低毒的杀菌剂，常用于防治谷物、蔬菜和水果中的植物病害。近年来其使用量显著增加，导致蔬菜中的残留量升高。本研究开发了一种灵敏、快速的免疫分析方法，利用金标和荧光标记的单克隆抗体（mAb）检测蔬菜样品中的PCM残留。在最佳条件下，荧光微球标记的单克隆抗体免疫层析试纸条的检测限（LOD）为1.67 ng/mL，目视LOD为50 ng/mL。批内准确度在94.98%至103.82%之间，变异系数（CV）为1.97%至8.26%。批间准确度在96.16%至102.51%之间，CV为4.62%至8.91%。金纳米颗粒标记的单克隆抗体免疫层析试纸条的目视检测范围为50至200 ng/g。该方法在实际蔬菜样品中表现优异，证实了其在各种基质中的适用性。这种双方法途径能够先用金试纸条快速筛选阴性样品，然后用荧光试纸条对阳性样品进行准确定量分析，从而提高效率并满足不同的检测需求。因此，该方法在实际应用中具有巨大的市场潜力。
