Jasińska Joanna Maria, Michalska Klaudia, Tkaczewska Joanna, Tkacz Katarzyna, Zakrzewski Arkadiusz, Galanty Agnieszka, Kamińska Iwona, Chmiel Maria J, Jamróz Ewelina
Department of Chemistry, University of Agriculture, ul. Balicka 122, 30-149 Kraków, Poland.
Department of Phytochemistry, Maj Institute of Pharmacology, Polish Academy of Sciences, ul. Smętna 12, 31-343 Kraków, Poland.
Molecules. 2025 Mar 25;30(7):1447. doi: 10.3390/molecules30071447.
Novel double-layer films based on furcellaran (FUR) and gelatin (GEL) with the addition of L. (PA) extract were used as active packaging for African catfish fillets. Films with PA extract have been shown to minimize the catfish spoilage effects, expressed as odor reduction compared to control samples; however, neither the films nor the PA extract exhibited antimicrobial activity against tested groups of microorganisms (fungi, lactic acid bacteria, and psychrotrops) or specified microorganisms (, , ). The tested films demonstrated antioxidant activity determined by the DPPH, ABTS, FRAP, CUPRAC and Folin-Ciocâlteu methods. Cytotoxicity analysis showed that the PA extract affected tested cell lines (PNT2-prostate epithelial cells, HepG2-human liver cells, HaCaT-normal human keratinocytes and Nty-hori 3-1) only to a small extent-the calculated IC values exceeded the maximal tested concentration of 500 µg/mL.
基于角叉菜聚糖（FUR）和明胶（GEL）并添加罗勒（PA）提取物的新型双层薄膜被用作非洲鲶鱼片的活性包装材料。含有PA提取物的薄膜已被证明能将鲶鱼的腐败影响降至最低，与对照样品相比，表现为气味减轻；然而，薄膜和PA提取物均未对测试的微生物组（真菌、乳酸菌和嗜冷菌）或特定微生物（、、）表现出抗菌活性。通过DPPH、ABTS、FRAP、CUPRAC和福林-西奥尔特法测定，测试薄膜具有抗氧化活性。细胞毒性分析表明，PA提取物仅在很小程度上影响测试的细胞系（PNT2-前列腺上皮细胞、HepG2-人肝细胞、HaCaT-正常人角质形成细胞和Nty-hori 3-1）——计算出的IC值超过了500μg/mL的最大测试浓度。
