Jiao Fangwen, Liu Tianyu, Wang Kaiwei, Li Shuai, Jiao Ruihua, Lin Wei
Department of Pathogen Biology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Jiangsu Key Laboratory of Medicinal Substance and Utilization of Fresh Chinese Medicine, Nanjing 210023, China.
Molecules. 2025 Mar 28;30(7):1502. doi: 10.3390/molecules30071502.
Two new decalin-tetramic acid hybrid metabolites, zopfiellamides C () and D () were isolated from the marine-derived fungus sp. NF666. The structure determination was accomplished on the basis of HRESIMS and NMR spectral data analyses including COSY, HSQC, HMBC, and NOESY experiments. Both isolated metabolites ( and ) exhibited significant growth inhibition against four clinically relevant bacterial strains with minimum inhibitory concentration (MIC) values of about 12.5 μΜ. Moreover, we proposed a plausible biosynthetic pathway of zopfiellamide D () in this work.
从海洋来源的真菌Zopfiella sp. NF666中分离出两种新的十氢化萘-四酰胺杂合代谢产物，佐菲拉酰胺C（）和D（）。通过高分辨电喷雾电离质谱（HRESIMS）和核磁共振（NMR）光谱数据分析，包括相关光谱（COSY）、异核单量子相干谱（HSQC）、异核多键相关谱（HMBC）和核Overhauser效应谱（NOESY）实验，完成了结构鉴定。两种分离得到的代谢产物（和）对四种临床相关细菌菌株均表现出显著的生长抑制作用，最低抑菌浓度（MIC）值约为12.5 μΜ。此外，在本研究中我们还提出了佐菲拉酰胺D（）合理的生物合成途径。
