College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830017, Xinjiang, PR China.
Xinjiang Key Laboratory of Hetian Characteristic Chinese Traditional Medicine Research, 848000 Hetian, PR China.
Ultrason Sonochem. 2024 Dec;111:107127. doi: 10.1016/j.ultsonch.2024.107127. Epub 2024 Oct 28.
Anchusa italica Retz. Flowers (Airfs) is a perennial herb belonging to the Boraginaceae family. A variety of pharmacological agents are employed in the treatment of a multitude of cardiovascular disorders. The study employs ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-Exactive Orbitrap-MS) to elucidate the complex chemical composition of these flowers, identifying a multitude of bioactive constituents. It is noteworthy that rosmarinic acid (RA) emerged as a predominant compound, a finding underscored by its high relative content of 39.8511% in the methanolic extract of the flowers. The innovative approach involved the use of a deep eutectic solvent (DES), a green extraction medium, which, when combined with ultrasonic assistance, resulted in a notable enhancement of the extraction yield of total polyphenols and RA. The optimization of this extraction process was achieved through a meticulous response surface methodology, which identified critical factors and their optimal conditions. These included a water content of 29.051 % and a material-liquid ratio of 20.961 mL/g. The optimal conditions were determined to be an extraction time of 35.725 min, an ultrasonic power of 240.342 W. In vitro antioxidant assays further substantiated the therapeutic potential of Anchusa italica Retz. flowers. RA demonstrated remarkable DPPH and ABTS radical scavenging capabilities, with rates exceeding 90%. The comprehensive evaluation value (CEV), derived from the total polyphenol content (TPC) and RA, was optimized under the aforementioned conditions, yielding a CEV of 222.452. The optimized extraction method presents a sustainable and efficient protocol for the isolation of bioactive compounds from natural sources, thereby underscoring the significance of this research in the field of natural product chemistry and ultrasonics Sonochemistry.
意大利琉璃苣花（Airfs）是一种属于紫草科的多年生草本植物。多种药理学制剂被用于治疗多种心血管疾病。本研究采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-Exactive Orbitrap-MS）技术，阐明了这些花的复杂化学成分，鉴定出多种生物活性成分。值得注意的是，迷迭香酸（RA）是一种主要化合物，其在花的甲醇提取物中的相对含量高达 39.8511%，这一发现得到了强调。本研究采用的创新方法涉及使用深共晶溶剂（DES）作为绿色提取介质，当与超声辅助结合使用时，可显著提高总多酚和 RA 的提取产率。通过详细的响应面法优化了该提取过程，确定了关键因素及其最佳条件。这些因素包括水含量为 29.051%，物料液比为 20.961 mL/g。最佳条件为提取时间 35.725 分钟，超声功率 240.342 W。体外抗氧化试验进一步证实了意大利琉璃苣花的治疗潜力。RA 表现出显著的 DPPH 和 ABTS 自由基清除能力，清除率超过 90%。根据总多酚含量（TPC）和 RA 计算出的综合评价值（CEV），在上述条件下得到优化，CEV 值为 222.452。该优化的提取方法为从天然来源中分离生物活性化合物提供了一种可持续且高效的方案，强调了该研究在天然产物化学和超声化学领域的重要性。
