Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Molecules. 2019 Jul 26;24(15):2718. doi: 10.3390/molecules24152718.
In this study, deep eutectic solvents (DESs) were used for the ultrasound-assisted extraction (UAE) of valuable bioactive compounds from Chinese wild rice ( spp.). To this end, 7 different choline chloride (CC)-based DESs were tested as green extraction solvents. Choline chloride/1,4-butanediol (DES-2) exhibited the best extraction efficiency in terms of parameters such as the total flavonoid content (TFC), total phenolic content (TPC), and free radical scavenging capacity (DPPH and ABTS). Subsequently, the UAE procedure using 76.6% DES-2 was also optimized: An extraction temperature of 51.2 °C and a solid-liquid ratio of 37.0 mg/mL were considered optimal by a Box-Behnken experiment. The optimized extraction procedure proved efficient for the extraction of 9 phenolic and 3 flavonoid compounds from Chinese wild rice as determined by quantification based on ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS). This work, thus, demonstrates the possibility of customizing green solvents that offer greater extraction capacity than that of organic solvents.
在这项研究中，深共晶溶剂（DESs）被用于从中国野生稻（ spp.）中超声辅助提取有价值的生物活性化合物。为此，测试了 7 种不同的氯化胆碱（CC）基 DESs 作为绿色提取溶剂。在总黄酮含量（TFC）、总酚含量（TPC）和自由基清除能力（DPPH 和 ABTS）等参数方面，胆碱氯化物/1,4-丁二醇（DES-2）表现出最佳的提取效率。随后，使用 76.6% DES-2 的 UAE 工艺也进行了优化：Box-Behnken 实验表明，最佳提取温度为 51.2°C，固液比为 37.0mg/mL。通过基于超高效液相色谱-三重四极杆串联质谱（UPLC-QqQ-MS）的定量分析，优化后的提取工艺被证明能够有效地从中国野生稻中提取 9 种酚类和 3 种黄酮类化合物。因此，这项工作证明了定制绿色溶剂的可能性，这些溶剂比有机溶剂具有更大的提取能力。
