牙周炎患者唾液中白细胞介素-1β、基质金属蛋白酶-8和基质金属蛋白酶-9的水平：一项系统评价和荟萃分析

Levels of IL-1β, MMP-8, and MMP-9 in the Saliva of Subjects With Periodontitis: A Systematic Review and Meta-Analysis.

作者信息

Alarcón-Sánchez Mario Alberto, Rodríguez-Montaño Ruth, Mosaddad Seyed Ali, Heboyan Artak

机构信息

Doctor of Science in Molecular Biology in Medicine Program, University Center of Health Sciences, University of Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, Mexico.

Institute of Research in Dentistry, Department of Integral Dental Clinics, University Center of Health Sciences, University of Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, Mexico.

出版信息

J Clin Lab Anal. 2025 May;39(10):e70040. doi: 10.1002/jcla.70040. Epub 2025 Apr 27.

DOI:10.1002/jcla.70040

PMID:40289477

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12089797/

Abstract

BACKGROUND AND OBJECTIVE

Proinflammatory cytokines and enzymes responsible for tissue destruction are important in the development of periodontitis. This study compared salivary concentrations of interleukin-1 beta (IL-1β), matrix metalloproteinases (MMP-8), and (MMP-9) in individuals with and without periodontitis to evaluate their diagnostic utility as potential biomarkers.

MATERIALS AND METHODS

A comprehensive search was performed across PubMed, Scopus, ScienceDirect, and Google Scholar, supplemented by manual searches in relevant journals up to January 2024. Eligibility criteria focused on human studies with defined diagnostic criteria for periodontitis and saliva samples analyzed for IL-1β, MMP-8, and MMP-9. Data were extracted to compare salivary levels of these markers between periodontitis patients and healthy controls. The Joanna Briggs Institute tool was used to evaluate the risk of bias and quality of the included studies. Statistical analysis employed a random effects model to calculate standardized mean differences and assess heterogeneity and publication bias.

RESULTS

The search yielded 122 articles, with 27 meeting the inclusion criteria. Fifteen percent of these studies presented a moderate risk of bias, while the remaining 85% exhibited a low risk of bias. The meta-analyses indicated significantly higher levels of IL-1β, MMP-8, and MMP-9 in the saliva of subjects with periodontitis compared to healthy individuals: IL-1β: Standardized Mean Difference (SMD) = 163.29 (95% CI = 104.64-221.95), p < 0.001; MMP-8: SMD = 282.22 (95% CI = 209.68-354.77), p < 0.001; MMP-9: SMD = 311.85 (95% CI = 179.64-444.05), p < 0.001.

CONCLUSION

Elevated salivary levels of IL-1β, MMP-8, and MMP-9 are linked to periodontitis.

摘要

背景与目的

促炎细胞因子和负责组织破坏的酶在牙周炎的发展过程中起着重要作用。本研究比较了患有和未患有牙周炎的个体唾液中白细胞介素-1β（IL-1β）、基质金属蛋白酶（MMP-8）和（MMP-9）的浓度，以评估它们作为潜在生物标志物的诊断效用。

材料与方法

对PubMed、Scopus、ScienceDirect和谷歌学术进行了全面检索，并辅以截至2024年1月在相关期刊上的手工检索。纳入标准侧重于有明确牙周炎诊断标准的人体研究以及对IL-1β、MMP-8和MMP-9进行分析的唾液样本。提取数据以比较牙周炎患者和健康对照之间这些标志物的唾液水平。使用乔安娜·布里格斯研究所工具评估纳入研究的偏倚风险和质量。统计分析采用随机效应模型来计算标准化均值差，并评估异质性和发表偏倚。

结果

检索共得到122篇文章，其中27篇符合纳入标准。这些研究中有15%存在中度偏倚风险，其余85%的偏倚风险较低。荟萃分析表明，与健康个体相比，牙周炎患者唾液中IL-1β、MMP-8和MMP-9的水平显著更高：IL-1β：标准化均值差（SMD）=163.29（95%置信区间=104.64-221.95），p<0.001；MMP-8：SMD=282.22（95%置信区间=209.68-354.77），p<0.001；MMP-9：SMD=311.85（95%置信区间=179.64-444.05），p<0.001。