2型糖尿病患者人胰腺分离胰岛中突出的基因表达改变。

Highlighted gene expression alteration in human pancreatic isolated islets in patients with type 2 diabetes.

作者信息

Mansouri Vahid, Arjmand Babak, Nejad Mohammad Rostami, Razzaghi Zahra, Rezaei-Tavirani Mostafa, Razi Farideh, Bandarian Fatemeh, Robati Reza M, Rezaei Mitra, Daneshimehr Fatemeh

机构信息

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

J Diabetes Metab Disord. 2025 Apr 23;24(1):107. doi: 10.1007/s40200-025-01611-x. eCollection 2025 Jun.

DOI:10.1007/s40200-025-01611-x

PMID:40291507

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12018653/

Abstract

OBJECTIVES

Type 2 diabetes is a complex disease characterized by progressive β-cell failure. The primary mechanism underlying this failure is the progressive loss of pancreatic β-cell function. The aim of this study is to identify the key gene expression changes in human pancreatic isolated islets of patients with type 2 diabetes.

METHODS

We extracted gene expression data in human pancreatic isolated islets of patients with type 2 diabetes and healthy controls from Gene Expression Omnibus (GEO) and analyzed it using GEO2R program. We then assessed the significant differentially expressed genes (DEGs) using protein-protein interaction (PPI) network analysis. The critical genes were enriched via gene ontology and discussed.

RESULTS

Among the 93 significant DEGs, five critical genes including ITGB2, APOE, BIRC5, GABRA2, and IL1B were emerged as key players in type 2 diabetes. Notably, "Alzheimer disease, type 4" was identified as a major class of biological terms altered in type 2 diabetes.

CONCLUSIONS

Our findings suggest that the introduced critical genes are potential targets for controlling type 2 diabetes. Furthermore, the crucial role of APOE as a link between type 2 diabetes and Alzheimer's disease or other cognitive disorders was confirmed.

摘要

目的

2型糖尿病是一种以进行性β细胞功能衰竭为特征的复杂疾病。这种功能衰竭的主要机制是胰腺β细胞功能的进行性丧失。本研究的目的是确定2型糖尿病患者人胰腺分离胰岛中的关键基因表达变化。

方法

我们从基因表达综合数据库（GEO）中提取了2型糖尿病患者和健康对照者人胰腺分离胰岛中的基因表达数据，并使用GEO2R程序进行分析。然后，我们通过蛋白质-蛋白质相互作用（PPI）网络分析评估了显著差异表达基因（DEG）。通过基因本体对关键基因进行富集并进行讨论。

结果

在93个显著的DEG中，包括整合素β2（ITGB2）、载脂蛋白E（APOE）、杆状病毒IAP重复序列5（BIRC5）、γ-氨基丁酸A受体α2亚基（GABRA2）和白细胞介素1β（IL1B）在内的5个关键基因成为2型糖尿病的关键因素。值得注意的是，“4型阿尔茨海默病”被确定为2型糖尿病中改变的主要生物学术语类别。