Waddell D R, Vogel G
Exp Cell Res. 1985 Aug;159(2):323-34. doi: 10.1016/s0014-4827(85)80006-9.
The predatory slime mold, D. caveatum, feeds upon other amoebae by phagocytosis. The D. caveatum amoebae begin feeding upon cells the same size or larger by nibbling pieces of cells. While feeding upon other amoebae as opposed to bacteria, they increase in size. This behavior resembles that of phagocytes in higher organisms. A novel method was used to follow the time course of phagocytosis. A lytic toxin, phallolysin, and mutants resistant to the toxin were utilized in an assay to separate the phagocytes from the prey cells. Since a broad spectrum of cells are sensitive to the toxin, the method has general applicability.
捕食性黏菌D. caveatum通过吞噬作用以其他变形虫为食。D. caveatum变形虫通过啃食细胞碎片开始以大小相同或更大的细胞为食。与以细菌为食相比，当以其他变形虫为食时，它们的体积会增大。这种行为类似于高等生物中的吞噬细胞。一种新方法被用于追踪吞噬作用的时间进程。在一项实验中，利用一种溶细胞毒素——鬼笔溶血素以及对该毒素有抗性的突变体，将吞噬细胞与猎物细胞分离。由于广泛的细胞类型对该毒素敏感，所以该方法具有普遍适用性。
