McKibbin J M, Smith E L, Månsson J E, Li Y T
Biochemistry. 1977 Mar 22;16(6):1223-8. doi: 10.1021/bi00625a030.
Glycolipids containing fucose (fucolipids) which carried human blood group A activity were isolated from a number of dog small intestines and analyzed. On the basis of sugar analysis, methylation, periodate oxidation, enzyme degradation, mass spectrometry, and immunologic studies, a structure is proposed for these substances. The ceramides of the dog fucolipids containing only hydroxylated fatty acids with 85% saturated and 15% monoenoic acids ranging from 16 to 25 carbon atoms. Sphingosine and phytosphingosine comprised 48% each of the long chain bases. An A-active fraction isolated from human small intestine was shown to have two components, one of which was immunologically distinct and the other identical with the dog intestinal fucolipids. The human fraction differed from the dog fucolipids in migration on thin-layer chromatography and contained two types of amino sugar substitution. It is proposed that the human fraction was composed of two fucolipids with incomplete structures.
从多条狗的小肠中分离并分析了含有携带人类 A 血型活性的岩藻糖的糖脂（岩藻糖脂）。基于糖类分析、甲基化、高碘酸盐氧化、酶降解、质谱分析和免疫学研究，提出了这些物质的结构。狗岩藻糖脂的神经酰胺仅含有羟基化脂肪酸，其中 85%为饱和脂肪酸，15%为单烯酸，碳原子数在 16 至 25 之间。鞘氨醇和植物鞘氨醇各占长链碱基的 48%。从人小肠中分离出的 A 活性组分显示有两种成分，其中一种在免疫学上不同，另一种与狗肠道岩藻糖脂相同。人组分在薄层色谱上的迁移与狗岩藻糖脂不同，并且含有两种类型的氨基糖取代。有人提出，人组分由两种结构不完整的岩藻糖脂组成。
