Smith E L, McKibbin J M, Karlsson K A, Pascher I, Samuelsson B E
Biochemistry. 1975 May 20;14(10):2120-4. doi: 10.1021/bi00681a012.
Glycolipids with blood group A activity isolated from human and dog small intestine have been characterized by mass spectrometry of intact lipid in methylated and in methylated and reduced (LiAiH4) form. Without degradative studies the glycolipids were conclusively shown to be hexaglycosyleramides with phytosphingosine as the major long-chain base and hydroxypalmitic acid as the major fatty acid. The exact sugar ratio was hexose-hexosamine-deoxyhexose 3:2:1 and the sequence established as hexosamine-[deoxyhexose-]hexose-hexosamine-hexose-hexose-ceramide. Evidence is presented that mass spectrometry can differential between type ) and type 2 saccharide chains.
从人和狗的小肠中分离出的具有A血型活性的糖脂，已通过对甲基化以及甲基化并还原（LiAlH4）形式的完整脂质进行质谱分析来表征。在没有降解研究的情况下，这些糖脂被确凿地证明是六糖基神经酰胺，其中植物鞘氨醇是主要的长链碱基，羟基棕榈酸是主要的脂肪酸。确切的糖比例为己糖-己糖胺-脱氧己糖3:2:1，序列确定为己糖胺-[脱氧己糖-]己糖-己糖胺-己糖-己糖-神经酰胺。有证据表明，质谱分析可以区分1型和2型糖链。
