Barth Isabel, Lee Hakho
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Light Sci Appl. 2025 Apr 28;14(1):177. doi: 10.1038/s41377-025-01866-2.
This review examines imaging-based nanophotonic biosensing and interferometric label-free imaging, with a particular focus on vesicle detection. It specifically compares dielectric and plasmonic metasurfaces for label-free protein and extracellular vesicle detection, highlighting their respective advantages and limitations. Key topics include: (i) refractometric sensing principles using resonant dielectric and plasmonic surfaces; (ii) state-of-the-art developments in both plasmonic and dielectric nanostructured resonant surfaces; (iii) a detailed comparison of resonance characteristics, including amplitude, quality factor, and evanescent field enhancement; and (iv) the relationship between sensitivity, near-field enhancement, and analyte overlap in different sensing platforms. The review provides insights into the fundamental differences between plasmonic and dielectric platforms, discussing their fabrication, integration potential, and suitability for various analyte sizes. It aims to offer a unified, application-oriented perspective on the potential of these resonant surfaces for biosensing and imaging, aiming at addressing topics of interest for both photonics experts and potential users of these technologies.
本综述探讨了基于成像的纳米光子生物传感和无标记干涉成像，特别关注囊泡检测。具体比较了用于无标记蛋白质和细胞外囊泡检测的介电超表面和等离子体超表面，突出了它们各自的优点和局限性。关键主题包括：（i）使用共振介电和等离子体表面的折射传感原理；（ii）等离子体和介电纳米结构共振表面的最新进展；（iii）共振特性的详细比较，包括幅度、品质因数和倏逝场增强；（iv）不同传感平台中灵敏度、近场增强和分析物重叠之间的关系。该综述深入探讨了等离子体和介电平台之间的根本差异，讨论了它们的制造、集成潜力以及对各种分析物尺寸的适用性。旨在为这些共振表面在生物传感和成像方面的潜力提供一个统一的、面向应用的视角，旨在解决光子学专家和这些技术的潜在用户感兴趣的话题。
