Celikaslan Hamdiye, Kaplan Davut Sinan, Orkmez Mustafa
Department of Physiology, Faculty of Medicine Gaziantep, Gaziantep University, Gaziantep, Turkey.
Department of Biochemistry, Gaziantep University Faculty of Medicine, Gaziantep, Turkey.
Sci Rep. 2025 Apr 28;15(1):14841. doi: 10.1038/s41598-025-99486-8.
This study aimed to investigate the effects of curcumin on neuroinflammation and cognitive function in a rat model of febrile convulsions (FC). This study was conducted on 10-day-old male Wistar rat pups, randomly assigned to four groups: Control, Curcumin, FC, and FC + Curcumin. FC were induced by placing the rats in a 44 °C water bath until convulsions occurred or for a maximum of 4 min. Curcumin (200 mg/kg/day) was administered intraperitoneally for seven consecutive days before FC induction. Neuroinflammation was assessed by measuring hippocampal and serum TNF-α and IL-1β levels using ELISA. Cognitive function was evaluated using the Morris Water Maze (MWM) test, where escape latency, swimming speed, and distance traveled were recorded, followed by a probe test on Day 5 to assess memory retention. Motor coordination was assessed using the Rotarod test, measuring latency to fall. Curcumin treatment significantly reduced hippocampal TNF-α levels in the FC model (FC group: 145.3 ± 12.1 pg/mL vs. FC + Curcumin group: 98.6 ± 9.4 pg/mL, p = 0.001). In the MWM test, curcumin-treated rats exhibited shorter escape latencies and improved spatial memory performance compared to the FC group (p < 0.05). By Day 4, the curcumin-treated group had a significantly shorter escape latency (FC group: 42.8 ± 5.9 s vs. FC + Curcumin group: 25.3 ± 4.1 s, p = 0.002) and traveled a shorter distance to reach the platform (FC group: 335.4 ± 22.8 cm vs. FC + Curcumin group: 192.6 ± 18.3 cm, p = 0.001). Curcumin administration significantly reduced FC termination time (FC group: 96.7 ± 7.4 s vs. FC + Curcumin group: 62.5 ± 5.9 s, p = 0.001). Curcumin exerts neuroprotective effects in FC by reducing hippocampal neuroinflammation and improving cognitive function. These findings suggest that curcumin could be a promising therapeutic agent in managing febrile seizure-related neuroinflammation and cognitive dysfunction. Further studies are warranted to explore its long-term efficacy and clinical applicability.
本研究旨在探讨姜黄素对高热惊厥（FC）大鼠模型神经炎症和认知功能的影响。本研究以10日龄雄性Wistar大鼠幼崽为对象，随机分为四组：对照组、姜黄素组、FC组和FC+姜黄素组。通过将大鼠置于44℃水浴中直至惊厥发生或最长4分钟来诱导FC。在诱导FC前连续7天腹腔注射姜黄素（200mg/kg/天）。通过酶联免疫吸附测定法（ELISA）测量海马和血清中肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平来评估神经炎症。使用莫里斯水迷宫（MWM）试验评估认知功能，记录逃避潜伏期、游泳速度和游动距离，然后在第5天进行探针试验以评估记忆保持情况。使用转棒试验评估运动协调性，测量掉落潜伏期。姜黄素治疗显著降低了FC模型中海马TNF-α水平（FC组：145.3±12.1pg/mL vs. FC+姜黄素组：98.6±9.4pg/mL，p=0.001）。在MWM试验中，与FC组相比，经姜黄素治疗的大鼠表现出更短的逃避潜伏期和改善的空间记忆表现（p<0.05）。到第4天，姜黄素治疗组的逃避潜伏期显著缩短（FC组：42.8±5.9秒 vs. FC+姜黄素组：25.3±4.1秒，p=0.002），到达平台的游动距离也更短（FC组：335.4±22.8厘米 vs. FC+姜黄素组：192.6±18.3厘米，p=0.001）。给予姜黄素显著缩短了FC终止时间（FC组：96.7±7.4秒 vs. FC+姜黄素组：62.5±5.9秒，p=0.001）。姜黄素通过减轻海马神经炎症和改善认知功能在FC中发挥神经保护作用。这些发现表明姜黄素可能是治疗高热惊厥相关神经炎症和认知功能障碍的一种有前景的治疗药物。有必要进一步研究以探索其长期疗效和临床适用性。
