Vajrychova Marie, Stranik Jaroslav, Kupcik Rudolf, Sadibolova Michaela, Kukla Rudolf, Bolehovska Radka, Kacerovsky Marian
Biomedical Research Centre, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
Department of Obstetrics and Gynecology, Faculty of Medicine in Hradec Kralove, University Hospital Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
Sci Rep. 2025 Apr 28;15(1):14814. doi: 10.1038/s41598-025-99969-8.
Preterm prelabour rupture of membranes (PPROM) complicated by intra-amniotic inflammation (IAI) represents a substantial proportion of preterm birth cases. Currently, IAI is frequently defined as amniotic fluid IL-6 concentration above 2,600 pg/mL. However, the amniotic fluid IL-6 concentration was never correlated with the global response of other proinflammatory proteins to the ongoing IAI. In this cross-sectional study, protein quantification was performed using mass spectrometry (MS) analysis followed by target quantification of selected proinflammatory proteins. Levels of amniotic fluid proteins determined by MS were put into the correlation with IL-6 concentration determined by electrochemiluminescence immunoassay method (ECLIA). In total, 925 proteins were efficiently quantified and differential expression analysis revealed 378 proteins upregulated towards IL-6 concentration above 10,000 pg/mL. Four proteins (LCN2, MMP8, MPO, and S100A12) were selected to verify the achieved results and IL-6 concentration of 10,000 pg/mL was determined as the cut-off value for global IAI response.
早产胎膜早破（PPROM）合并羊膜腔内炎症（IAI）占早产病例的很大比例。目前，IAI常被定义为羊水白细胞介素-6（IL-6）浓度高于2600 pg/mL。然而，羊水IL-6浓度从未与其他促炎蛋白对持续IAI的整体反应相关联。在这项横断面研究中，采用质谱（MS）分析进行蛋白质定量，随后对选定的促炎蛋白进行靶向定量。通过MS测定的羊水蛋白水平与通过电化学发光免疫分析法（ECLIA）测定的IL-6浓度进行相关性分析。总共有效定量了925种蛋白质，差异表达分析显示，当IL-6浓度高于10000 pg/mL时，有378种蛋白质上调。选择了四种蛋白质（LCN2、MMP8、MPO和S100A12）来验证所得结果，并将10000 pg/mL的IL-6浓度确定为IAI整体反应的临界值。
