Blesl Andreas, Binder Lukas, Halwachs Bettina, Baumann-Durchschein Franziska, Fürst Stefan, Constantini-Kump Patrizia, Wenzl Heimo, Gorkiewicz Gregor, Högenauer Christoph
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
Institute for Pharmaceutical Sciences, University of Graz, Graz, Austria.
Inflamm Bowel Dis. 2025 Jul 7;31(7):2007-2018. doi: 10.1093/ibd/izaf053.
The fecal microbiome of patients with inflammatory bowel diseases (IBD) is characterized by longitudinal variability. It remains unknown if this is caused by decreased resilience of the microbiome to external factors. We investigated the influence of osmotic diarrhea induced by bowel preparation as a disruptive factor on the fecal microbiome in IBD patients and healthy comparators.
We conducted a prospective, single-center study including IBD patients and healthy controls scheduled for colonoscopy with uniform bowel preparation. Fecal samples were collected at 7 time points prior, during, and until 3 months after the intervention. 16S rRNA was isolated from stool and sequenced using the Illumina technique.
Twenty-two IBD patients and 17 healthy controls were included in the study. Baseline diversity was higher in healthy controls. Bowel preparation longitudinally decreased alpha diversity and altered beta diversity and taxonomic composition in both groups. Alterations were more pronounced in healthy controls, and the microbial composition converged between the 2 groups. Bowel preparation resulted in an increased relative abundance of Anaerostipes and Coprococcus in both groups and in decreased relative abundance of Bifidobacterium and Clostridium sensu stricto in IBD patients and of Eubacterium hallii group and Ruminococcus in healthy controls. Changes largely restored to baseline composition within 1 week in both groups and remained stable thereafter.
Bowel preparation induced reversible short-term microbial perturbations which were less pronounced in IBD patients than in healthy comparators suggesting that a single external disruptive factor may have less impact on an already altered fecal microbiome.
炎症性肠病（IBD）患者的粪便微生物群具有纵向变异性。目前尚不清楚这是否是由于微生物群对外界因素的恢复力下降所致。我们研究了肠道准备引起的渗透性腹泻作为干扰因素对IBD患者和健康对照者粪便微生物群的影响。
我们进行了一项前瞻性单中心研究，纳入计划进行结肠镜检查且肠道准备统一的IBD患者和健康对照者。在干预前、干预期间及干预后3个月内的7个时间点采集粪便样本。从粪便中分离16S rRNA，并使用Illumina技术进行测序。
本研究纳入了22例IBD患者和17名健康对照者。健康对照者的基线多样性更高。肠道准备在纵向降低了两组的α多样性，改变了β多样性和分类组成。健康对照者的变化更为明显，两组之间的微生物组成趋于一致。肠道准备导致两组中厌氧棒状菌属和粪球菌属的相对丰度增加，IBD患者中双歧杆菌属和严格意义上的梭菌属以及健康对照者中土栖杆菌属组和瘤胃球菌属的相对丰度降低。两组的变化在1周内基本恢复到基线组成，此后保持稳定。
肠道准备引起可逆的短期微生物扰动，IBD患者的扰动不如健康对照者明显，这表明单一的外部干扰因素可能对已经改变的粪便微生物群影响较小。
