Nguyen Thi Le Na, Nguyen Ngoc Huyen, Sai Cong Doanh, Bui Thi Viet Ha, Do Huu Quyet, Vu Thi Thao, Vu Duy Minh
Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi, Vietnam.
Faculty of Physics, VNU University of Science, Vietnam National University, Hanoi, Vietnam.
Sci Prog. 2025 Apr-Jun;108(2):368504251338628. doi: 10.1177/00368504251338628. Epub 2025 Apr 29.
ObjectiveCurcumin is a polyphenol extracted from the roots of . It is a multifunctional biological substance that has notable antioxidant, anti-inflammatory, anti-cancer, and antibacterial properties, as reported in numerous previous papers. However, curcumin has limited bioavailability due to its low water solubility. Furthermore, pH, temperature, and light all have the potential to decrease curcumin's biopharmaceutical activities. Silver nanoparticles have excellent antibacterial characteristics and are used in various applications. The main objective of this work was to synthesize the gelatin microgel-stabilized silver nanoparticles loaded with curcumin (GelCurAg complex) to increase the biopharmaceutical activities of the complex.MethodsNumerous techniques, including UV-vis spectroscopy, FTIR spectroscopy, Zetasizer Nano instruments, TEM images, and XDR patterns, were used for investigating the characterization of the GelCurAg complex. The various methods, such as the DPPH antioxidant assay, cytotoxicity analysis, and the agar-well diffusion method, were used for the analysis of the biopharmaceutical activities of the GelCurAg complex.ResultsWe successfully synthesized and characterized the GelCurAg complex, consisting of gelatin, curcumin, and silver nanoparticle biofilms using polyvinylpyrrolidone. The average nanoparticle size was 50 nm. The GelCurAg complex improved water solubility and dispersion compared with the single curcumin component. The IC value for antioxidant activity was 4.87 μg/mL, and for antiproliferation on SK-MEL-28, it was 3.98 µg/mL. GelCurAg solution provided remarkable antibacterial activities against four bacterial strains: , and . The GelCurAg complex showed better antibacterial activities than the single curcumin component, with the antibacterial inhibition zone in the range of 0.9--1.6 cm.ConclusionsThe GelCurAg complex enhanced the solubility and dispersion of curcumin in water while increasing its antibacterial activity compared to curcumin. The GelCurAg complex has excellent biopharmaceutical properties to suggest its various applications in the future.
目的
姜黄素是从姜黄根中提取的一种多酚。如众多先前论文所报道，它是一种具有显著抗氧化、抗炎、抗癌和抗菌特性的多功能生物物质。然而，姜黄素由于其低水溶性，生物利用度有限。此外，pH值、温度和光照都有可能降低姜黄素的生物制药活性。银纳米颗粒具有优异的抗菌特性，被用于各种应用。这项工作的主要目的是合成负载姜黄素的明胶微凝胶稳定化银纳米颗粒（GelCurAg复合物），以提高该复合物的生物制药活性。
方法
使用了多种技术，包括紫外可见光谱、傅里叶变换红外光谱、Zetasizer Nano仪器、透射电子显微镜图像和X射线衍射图谱，来研究GelCurAg复合物的特性。采用了各种方法，如DPPH抗氧化测定、细胞毒性分析和琼脂孔扩散法，来分析GelCurAg复合物的生物制药活性。
结果
我们成功合成并表征了由明胶、姜黄素和使用聚乙烯吡咯烷酮的银纳米颗粒生物膜组成的GelCurAg复合物。纳米颗粒的平均尺寸为50纳米。与单一姜黄素成分相比，GelCurAg复合物提高了水溶性和分散性。抗氧化活性的IC值为4.87μg/mL，对SK-MEL-28细胞的抗增殖IC值为3.98μg/mL。GelCurAg溶液对四种细菌菌株：[此处原文缺失细菌菌株名称]、[此处原文缺失细菌菌株名称]和[此处原文缺失细菌菌株名称]表现出显著的抗菌活性。GelCurAg复合物比单一姜黄素成分表现出更好的抗菌活性，抗菌抑制圈在0.9 - 1.6厘米范围内。
结论
GelCurAg复合物增强了姜黄素在水中的溶解度和分散性，同时与姜黄素相比提高了其抗菌活性。GelCurAg复合物具有优异的生物制药特性，表明其在未来有各种应用前景。
