Chansoria Parth, Winkelbauer Michael, Zhang Shipin, Janiak Jakub, Liu Hao, Boev Dimitar, Morandi Andrea, Grange Rachel, Zenobi-Wong Marcy
Department of Health Sciences and Technology, Institute for Biomechanics, Tissue Engineering and Biofabrication Group, ETH Zürich, 8093, Switzerland.
Department of Physics, Institute for Quantum Electronics, Optical Nanomaterial Group, ETH Zürich, 8093, Switzerland.
Adv Mater. 2025 Jul;37(27):e2419350. doi: 10.1002/adma.202419350. Epub 2025 Apr 29.
Light-based biofabrication techniques have revolutionized the field of tissue engineering and regenerative medicine. Specifically, the projection of structured light, where the spatial distribution of light is controlled at both macro and microscale, has enabled precise fabrication of complex three dimensional structures with high resolution and speed. However, despite tremendous progress, biofabrication processes are mostly limited to benchtop devices which limit the flexibility in terms of where the fabrication can occur. Here, a Fiber-assisted Structured Light (FaSt-Light) projection apparatus for rapid in situ crosslinking of photoresins is demonstrated. This approach uses image-guide fiber bundles which can project bespoke images at multiple wavelengths, enabling flexibility and spatial control of different photoinitiation systems and crosslinking chemistries and also the location of fabrication. Coupling of different sizes of fibers and different lenses attached to the fibers to project small (several mm) or large (several cm) images for material crosslinking is demonstrated. FaSt-Light allows control over the cross-section of the crosslinked resins and enables the introduction of microfilaments which can further guide cellular infiltration, differentiation, and anisotropic matrix production. The proposed approach can lead to a new range of in situ biofabrication techniques which improve the translational potential of photofabricated tissues and grafts.
基于光的生物制造技术彻底改变了组织工程和再生医学领域。具体而言，结构化光的投射，即光的空间分布在宏观和微观尺度上都得到控制，使得能够以高分辨率和速度精确制造复杂的三维结构。然而，尽管取得了巨大进展，但生物制造过程大多局限于台式设备，这限制了制造地点的灵活性。在此，展示了一种用于光致树脂快速原位交联的光纤辅助结构化光（FaSt-Light）投射装置。这种方法使用图像引导光纤束，其可以在多个波长下投射定制图像，从而实现对不同光引发系统和交联化学以及制造位置的灵活性和空间控制。展示了将不同尺寸的光纤和连接到光纤上的不同透镜耦合，以投射小（几毫米）或大（几厘米）的图像用于材料交联。FaSt-Light允许控制交联树脂的横截面，并能够引入微丝，这可以进一步引导细胞浸润、分化和各向异性基质产生。所提出的方法可以带来一系列新的原位生物制造技术，提高光制造组织和移植物的转化潜力。
