Jones Lewis S, Filippi Miriam, Michelis Mike Yan, Balciunaite Aiste, Yasa Oncay, Aviel Gal, Narciso Maria, Freedrich Susanne, Generali Melanie, Tzahor Eldad, Katzschmann Robert K
Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, Zurich, 8092, Switzerland.
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel.
Adv Sci (Weinh). 2024 Dec;11(47):e2404509. doi: 10.1002/advs.202404509. Epub 2024 Oct 7.
Biofabricating 3D cardiac tissues that mimic the native myocardial tissue is a pivotal challenge in tissue engineering. In this study, we fabricate 3D cardiac tissues with controlled, multidirectional cellular alignment and directed or twisting contractility. We show that multidirectional filamented light can be used to biofabricate high-density (up to 60 × 10 cells mL) tissues, with directed uniaxial contractility (3.8x) and improved cell-to-cell connectivity (1.6x gap junction expression). Furthermore, by using multidirectional light projection, we can partially overcome cell-induced light attenuation, and fabricate larger tissues with multidirectional cellular alignment. For example, we fabricate a tri-layered myocardium-like tissue and a bi-layered tissue with torsional contractility. The approach provides a new strategy to rapidly fabricate aligned cardiac tissues relevant to regenerative medicine and biohybrid robotics.
生物制造模仿天然心肌组织的三维心脏组织是组织工程中的一项关键挑战。在本研究中，我们制造了具有可控的多向细胞排列以及定向或扭转收缩性的三维心脏组织。我们表明，多向丝状光可用于生物制造高密度（高达60×10个细胞/毫升）组织，具有定向单轴收缩性（3.8倍）和改善的细胞间连接性（缝隙连接表达增加1.6倍）。此外，通过使用多向光投影，我们可以部分克服细胞引起的光衰减，并制造具有多向细胞排列的更大组织。例如，我们制造了具有扭转收缩性的三层心肌样组织和双层组织。该方法为快速制造与再生医学和生物杂交机器人相关的排列心脏组织提供了一种新策略。
