Satchasataporn Khomson, Khunbutsri Duangdaow, Chopjitt Peechanika, Sutjarit Samak, Pan-Utai Wanida, Meekhanon Nattakan
Department of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Antibiotics (Basel). 2025 Apr 3;14(4):377. doi: 10.3390/antibiotics14040377.
Multidrug-resistant (MDR) bacteria, including extended-spectrum beta-lactamase (ESBL)-producing , in companion animals pose a growing public health concern due to the close interactions between pets and humans. This study aimed to investigate antimicrobial resistance patterns and the prevalence of ESBL-producing isolated from healthy dogs in Thailand, as well as the potential of algal extracts obtained through ethanol extraction and enzymatic hydrolysis as alternative antimicrobial agents against these drug-resistant organisms. Antimicrobial susceptibility testing was performed on 43 isolates from healthy dogs. ESBL production was confirmed using standard phenotypic methods, and resistance genes were detected by PCR. The algal extracts were tested for antibacterial activity against MDR isolates. Among the 43 isolates, 67.44% were classified as MDR, with high resistance rates observed for ampicillin (79.07%), tetracycline (65.12%), and ciprofloxacin (62.79%), highlighting significant antimicrobial resistance concerns. Of the MDR isolates, 31.03% (9/29) were confirmed as ESBL producers. Gene analysis revealed as the most prevalent gene (53.49%), followed by (9.30%), while was detected in a single isolate resistant only to ampicillin and was absent in all MDR strains. Ethanol extracts of and demonstrated inhibitory effects against MDR . MDR and ESBL-producing are prevalent in healthy dogs, posing a potential public health risk. Algal extracts from and show promise as alternative antimicrobials. Further research is necessary to optimize their efficacy and investigate their in vivo applications, including clinical and environmental settings.
包括产超广谱β-内酰胺酶（ESBL）细菌在内的多重耐药（MDR）细菌在伴侣动物中日益引起公共卫生关注，因为宠物与人类之间存在密切互动。本研究旨在调查泰国健康犬中分离出的抗菌药物耐药模式及产ESBL细菌的流行情况，以及通过乙醇提取和酶水解获得的藻类提取物作为针对这些耐药菌的替代抗菌剂的潜力。对从健康犬分离出的43株细菌进行了药敏试验。使用标准表型方法确认ESBL的产生，并通过PCR检测耐药基因。测试了藻类提取物对MDR分离株的抗菌活性。在43株分离株中，67.44%被归类为MDR，氨苄西林（79.07%）、四环素（65.12%）和环丙沙星（62.79%）的耐药率较高，突出了显著的抗菌药物耐药问题。在MDR分离株中，31.03%（9/29）被确认为产ESBL菌。基因分析显示blaCTX-M为最常见基因（53.49%）,其次是blaTEM（9.30%），而blaSHV仅在一株仅对氨苄西林耐药的分离株中检测到，在所有MDR菌株中均未出现。小球藻和杜氏盐藻的乙醇提取物对MDR分离株显示出抑制作用。MDR和产ESBL细菌在健康犬中普遍存在，构成潜在的公共卫生风险。小球藻和杜氏盐藻的藻类提取物有望作为替代抗菌剂。有必要进一步研究以优化其疗效并研究其在体内的应用，包括临床和环境环境。
