Bonnard Fernanda Cruz, Guimarães Luciana, Teixeira Izabel Mello, Freire Sandryelle Mercês, Maia Alessandra, Pinto Patrícia Câmara de Castro Abreu, Blanchart Thais Veiga, Penna Bruno
Laboratório de Cocos Gram Positivos, Instituto Biomédico, Universidade Federal Fluminense (UFF), Niterói 24210-130, Brazil.
Antibiotics (Basel). 2025 Apr 16;14(4):409. doi: 10.3390/antibiotics14040409.
spp. and () are Gram-positive cocci that inhabit mammals' and birds' skin and mucous membranes, part of the microbiota. An imbalance in local immunity can increase colonization, resulting in various infections. Inappropriate use of antimicrobials leads to Staphylococci and becoming resistant to conventional treatments. The transmission of methicillin-resistant staphylococci and (MRS and MRMs, respectively) between humans and animals is still underreported in Brazil. this study aimed to describe the frequency, distribution, resistance pattern, and evaluation of potential sharing of MRS and MRMs in isolates from asymptomatic dogs and their owners in Rio de Janeiro. Samples from 50 asymptomatic dogs and 34 from their owners were collected. Isolates were identified by mass spectrometry. The gene was confirmed by conventional PCR. Antimicrobial activity of samples that carried the gene was evaluated by disk diffusion method. In this study, MRS and MRMs were analyzed in 50 dogs and their owners (34) by identifying strains carrying the gene. A total of 185 strains were isolated. The gene was found in 33.5% of the isolates. The most prevalent species carrying the gene was (33.9%). MRMs showed 14.5%. Fourteen dogs had the same strain carrying the gene as their owners. Of these, 50% exhibited the same antimicrobial resistance pattern, determined by the disk diffusion. The highest percentage of resistance observed in the MRS isolated from dogs and the owners was to Erythromycin (51.3% and 56.5%, respectively). The presence of methicillin-resistant staphylococci is worrisome because there is the potential to transfer these strains between dogs and humans. These strains may act as a reservoir of resistance genes.
葡萄球菌属和（）是革兰氏阳性球菌，栖息于哺乳动物和鸟类的皮肤及黏膜，是微生物群的一部分。局部免疫力失衡会增加定植，导致各种感染。抗菌药物的不当使用会使葡萄球菌和对传统治疗产生耐药性。在巴西，耐甲氧西林葡萄球菌和（分别为MRS和MRMs）在人和动物之间的传播情况仍报道不足。本研究旨在描述里约热内卢无症状犬及其主人分离株中MRS和MRMs的频率、分布、耐药模式以及潜在共享情况的评估。收集了50只无症状犬及其34名主人的样本。通过质谱法鉴定分离株。通过常规PCR确认mecA基因。采用纸片扩散法评估携带mecA基因样本的抗菌活性。在本研究中，通过鉴定携带mecA基因的菌株，对50只犬及其34名主人中的MRS和MRMs进行了分析。共分离出185株菌株。在33.5%的分离株中发现了mecA基因。携带mecA基因最常见的菌种是（33.9%）。MRMs占14.5%。14只犬与其主人携带相同的携带mecA基因的菌株。其中，50%表现出相同的抗菌耐药模式，由纸片扩散法确定。从犬和主人分离出的MRS中观察到的最高耐药百分比是对红霉素（分别为51.3%和56.5%）。耐甲氧西林葡萄球菌的存在令人担忧，因为这些菌株有可能在犬和人之间传播。这些菌株可能作为耐药基因的储存库。
