Zhang Ruyan, Wang Yong, Kang Yichen, Du Yunyun, Wang Xingxing, Jiao Shujuan, Yang Xinyu, Liu Yuhui, Qin Shuhao, Zhang Weina
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070 , China.
Potato Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China.
Plant Cell Rep. 2025 Apr 29;44(5):109. doi: 10.1007/s00299-025-03496-9.
Transcriptome proteome association analysis screened candidate DEGs, DEPs, and DEGs/DEPs associated with potato response to drought, alkali, and combined stresses. Overexpression of StCOMT1 enhances potato drought and alkali tolerance. Drought and salinity have severely impeded potato (Solanum tuberosum L.) growth and development, significantly reducing global potato production. However, the molecular mechanisms regulating the combined drought and alkali stress process are not fully understood. This study compared the mRNA and protein expression profiles of potato under drought (PEG-6000), alkali (NaHCO), and combined (PEG-6000 + NaHCO) stresses by transcriptome and TMT proteomics sequencing to investigate the common or specific responses of 'Atlantic' potato to single and combined stresses of drought and alkali were preliminarily explored. It was found that 2215 differentially expressed genes (DEGs) and 450 differentially expressed proteins (DEPs) were jointly identified under drought, alkali, and combined stresses. Under drought, alkali, and combined stresses, 234, 185, and 246 DEGs/DEPs were identified, respectively. These DEGs, DEPs, and DEGs/DEPs identified revealed the potential roles of several signaling and metabolic pathways in mediating drought and alkali stress tolerance, including plant hormone signaling, MAPK signaling pathway, phenylpropanoid biosynthesis, and glutathione metabolism. Caffeic acid-O-methyltransferase (COMT) is an essential methylating enzyme in the phenylpropane biosynthetic pathway, which is involved in lignin synthesis and plays an important role in protecting plants from abiotic stresses. In this study, we investigated the changes in physiologic characteristics, such as growth, antioxidant defense, osmotic regulation and lignin accumulation, in overexpressing StCOMT1 (PT0001512/M0ZIL7) transgenic potato after stress. It proved that the gene has the function of adapting to drought and alkali stress, and provided a theoretical basis for further research on the resistance mechanism of the gene in drought and alkali tolerance in potato.
转录组-蛋白质组关联分析筛选出了与马铃薯对干旱、盐碱及复合胁迫响应相关的候选差异表达基因(DEGs)、差异表达蛋白(DEPs)以及差异表达基因/差异表达蛋白(DEGs/DEPs)。StCOMT1的过表达增强了马铃薯的耐旱性和耐碱性。干旱和盐害严重阻碍了马铃薯(Solanum tuberosum L.)的生长发育,显著降低了全球马铃薯产量。然而,调控干旱和盐碱复合胁迫过程的分子机制尚未完全明确。本研究通过转录组和TMT蛋白质组测序比较了马铃薯在干旱(PEG-6000)、盐碱(NaHCO)及复合(PEG-6000 + NaHCO)胁迫下的mRNA和蛋白质表达谱,初步探究了‘大西洋’马铃薯对干旱和盐碱单一及复合胁迫的共同或特异性响应。研究发现,在干旱、盐碱及复合胁迫下共鉴定出2215个差异表达基因(DEGs)和450个差异表达蛋白(DEPs)。在干旱、盐碱及复合胁迫下,分别鉴定出234、185和246个DEGs/DEPs。这些鉴定出的DEGs、DEPs和DEGs/DEPs揭示了几种信号转导和代谢途径在介导干旱和盐碱胁迫耐受性中的潜在作用,包括植物激素信号转导、MAPK信号通路、苯丙烷生物合成和谷胱甘肽代谢。咖啡酸-O-甲基转移酶(COMT)是苯丙烷生物合成途径中的一种关键甲基化酶,参与木质素合成,并在保护植物免受非生物胁迫中发挥重要作用。在本研究中,我们研究了过表达StCOMT1(PT0001512/M0ZIL7)的转基因马铃薯在胁迫后的生理特性变化,如生长、抗氧化防御、渗透调节和木质素积累。结果证明该基因具有适应干旱和盐碱胁迫的功能,为进一步研究该基因在马铃薯耐旱和耐碱抗性机制方面提供了理论依据。