Mihai Mara-Mădălina, Bălăceanu-Gurău Beatrice, Holban Alina Maria, Ilie Cornelia-Ioana, Sima Romina Maria, Gurău Cristian-Dorin, Dițu Lia-Mara
Department of Oncologic Dermatology, "Elias" Emergency University Hospital, "Carol Davila" University of Medicine and Pharmacy, 020021 Bucharest, Romania.
Research Institute of the University of Bucharest, Department of Botany-Microbiology, Faculty of Biology, University of Bucharest, 050663 Bucharest, Romania.
Biomedicines. 2025 Apr 14;13(4):962. doi: 10.3390/biomedicines13040962.
Disruption of the natural balance of the skin microbiota can impair wound healing and contribute to chronic infections. Identifying the bacterial species involved and understanding their antimicrobial susceptibility profiles are essential for guiding treatment, especially given the growing threat of antibiotic resistance. This study characterized the virulence and antibiotic resistance phenotypes of 43 bacterial strains isolated from chronic wounds. The antimicrobial activity of selected essential oils (sandalwood, ylang-ylang, sage, cajeput, and juniper), pharmaceutical products (propolis tinctures, usnic acid), and probiotic lactic acid bacteria strains ( spp., ) was assessed using qualitative and quantitative assays, including MIC, MICBA, and co-culture evaluations. Gram-positive strains were more sensitive to essential oils than Gram-negative strains, with sandalwood, ylang-ylang, and propolis tincture showing the strongest antibacterial effects. These agents also showed significant biofilm inhibition. Probiotic strains exhibited antimicrobial activity against and , with and being particularly effective in reducing bacterial growth and adhesion in vitro. Essential oils and probiotic strains demonstrate promising antimicrobial effects against chronic wound pathogens and may serve as alternative or adjunctive treatments to antibiotics. Further clinical research and standardization are necessary to establish their safety, efficacy, and optimal application protocols.
皮肤微生物群自然平衡的破坏会损害伤口愈合并导致慢性感染。鉴于抗生素耐药性的威胁日益增加，确定其中涉及的细菌种类并了解它们的抗菌敏感性概况对于指导治疗至关重要。本研究对从慢性伤口分离出的43株细菌菌株的毒力和抗生素耐药表型进行了表征。使用定性和定量分析方法，包括最低抑菌浓度（MIC）、最低抑菌浓度杀菌活性（MICBA）和共培养评估，评估了所选精油（檀香、依兰、鼠尾草、白千层和杜松）、药品（蜂胶酊剂、松萝酸）和益生菌乳酸菌菌株（ 种、 种）的抗菌活性。革兰氏阳性菌株比革兰氏阴性菌株对精油更敏感，檀香、依兰和蜂胶酊剂显示出最强的抗菌效果。这些制剂还表现出显著的生物膜抑制作用。益生菌菌株对 种和 种表现出抗菌活性，其中 种和 种在体外减少细菌生长和粘附方面特别有效。精油和益生菌菌株对慢性伤口病原体显示出有前景的抗菌作用，可作为抗生素的替代或辅助治疗。需要进一步的临床研究和标准化来确定它们的安全性、有效性和最佳应用方案。
