Networking Biomedical Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 06006 Badajoz, Spain.
Department of Biomedical Science, Area of Microbiology, University of Extremadura, 06006 Badajoz, Spain.
Molecules. 2020 Jul 22;25(15):3318. doi: 10.3390/molecules25153318.
Propolis is a natural product obtained from hives. Its chemical composition varies depending on the flora of its surroundings, but nevertheless, common for all types of propolis, they all exhibit remarkable biological activities. The aim of this study was to investigate the chemical composition and antimicrobial activity of a novel Spanish Ethanolic Extract of Propolis (SEEP). It was found that this new SEEP contains high amounts of polyphenols (205 ± 34 mg GAE/g), with unusually more than half of this of the flavonoid class (127 ± 19 mg QE/g). Moreover, a detailed analysis of its chemical composition revealed the presence of olive oil compounds (Vanillic acid, 1-Acetoxypinoresinol, -HPEA-EA and 3,4-DHPEA-EDA) never detected before in propolis samples. Additionally, relatively high amounts of ferulic acid and quercetin were distinguished, both known for their important therapeutic benefits. Regarding the antimicrobial properties of SEEP, the minimal inhibitory and bactericidal concentrations (MIC and MBC) against strains were found at the concentrations of 240 and 480 µg/mL, respectively. Importantly, subinhibitory concentrations were also found to significantly decrease bacterial growth. Therefore, the results presented here uncover a new type of propolis rich in flavonoids with promising potential uses in different areas of human health.
蜂胶是一种从蜂巢中获得的天然产物。其化学成分因周围植物群的不同而有所差异，但所有类型的蜂胶都具有显著的生物活性。本研究旨在研究一种新型西班牙蜂胶乙醇提取物（SEEP）的化学成分和抗菌活性。结果发现，这种新型 SEEP 含有大量的多酚（205±34mgGAE/g），其中黄酮类化合物的含量异常高，超过一半（127±19mgQE/g）。此外，对其化学成分的详细分析表明，该蜂胶中含有从未在蜂胶样品中检测到的橄榄油化合物（香草酸、1-乙酰氧基松柏醇、-HPEA-EA 和 3,4-DHPEA-EDA）。此外，还区分出了相对较高含量的阿魏酸和槲皮素，这两种物质都因其重要的治疗益处而闻名。关于 SEEP 的抗菌特性，发现对 菌株的最小抑菌和杀菌浓度（MIC 和 MBC）分别在 240 和 480μg/mL 的浓度下。重要的是，亚抑菌浓度也被发现可显著抑制细菌生长。因此，这里提出的结果揭示了一种富含类黄酮的新型蜂胶，具有在人类健康的不同领域中具有广阔的应用前景。
