Carlsson J, Kujala U, Edlund M B
Infect Immun. 1985 Sep;49(3):674-8. doi: 10.1128/iai.49.3.674-678.1985.
Streptococcus mutans NCTC 10449 and Escherichia coli K-12 strain 37 were grown under aerobic and anaerobic conditions. In cell extracts of both strains, pyruvate dehydrogenase activity dependent on thiamine pyrophosphate, coenzyme A, and NAD was shown. The enzyme was induced by pyruvate in the growth medium, and there was higher activity in aerobically grown cells than in anaerobically grown cells. Acetyl phosphate was a potent inhibitor of the activity. This inhibition was partly overcome by inorganic phosphate.
变形链球菌NCTC 10449和大肠杆菌K-12菌株37在需氧和厌氧条件下培养。在这两种菌株的细胞提取物中，均显示出依赖硫胺素焦磷酸、辅酶A和NAD的丙酮酸脱氢酶活性。该酶由生长培养基中的丙酮酸诱导，需氧生长细胞中的活性高于厌氧生长细胞。乙酰磷酸是该活性的有效抑制剂。无机磷酸盐可部分克服这种抑制作用。
