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中温及低温亚硝化-厌氧氨氧化反应器的微生物群落结构:核心微生物组的分布及功能作用

Microbial Community Structure of Mesophilic and Low-temperature Partial Nitrification-anammox Reactors: Distribution and Functional Roles of the Core Microbiome.

作者信息

Oshiki Mamoru, Takahashi Kohei, Kawasaki Seiya, Choi Hyungmin, Park Jihye, Kim Kwiyong, Bae Hyokwan, Okabe Satoshi, Lee Changsoo

机构信息

Division of Environmental Engineering, Faculty of Engineering, Hokkaido University.

JSPS Postdoctoral Fellow, Hokkaido University.

出版信息

Microbes Environ. 2025;40(2). doi: 10.1264/jsme2.ME25001.

DOI:10.1264/jsme2.ME25001
PMID:40301070
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12213063/
Abstract

Microbial community structures in mesophilic and low-temperature anammox and partial nitrification-anammox reactors were exami-ned by a 16S rRNA-gene amplicon sequencing ana-lysis. The anammox bacterium, Jettenia sp., was dominant, and nitrifying bacteria, including Nitrosomonas sp. (aerobic ammonia-oxidizing bacterium) and Nitrospira sp., (nitrite-oxidizing bacterium) coexisted in the bioreactors. Core coexisting bacteria, such as Sulfurisoma sp. and Zeimonas sp., showed oxygen-scavenging and NO reduction potentials. Sulfurisoma-related bacteria are distributed across wastewater treatment plants worldwide, particularly in denitrification systems. These results underscore the ecological and functional importance of microbial consortia in enhancing nitrogen removal efficiency.

摘要

通过16S rRNA基因扩增子测序分析,研究了中温及低温厌氧氨氧化和部分硝化-厌氧氨氧化反应器中的微生物群落结构。厌氧氨氧化菌Jettenia sp.占主导地位,包括亚硝化单胞菌属(好氧氨氧化菌)和硝化螺菌属(亚硝酸盐氧化菌)在内的硝化细菌在生物反应器中共存。核心共存细菌,如硫杆菌属和泽氏菌属,表现出氧清除和NO还原潜力。与硫杆菌属相关的细菌分布在全球各地的污水处理厂,特别是在反硝化系统中。这些结果强调了微生物群落对提高脱氮效率的生态和功能重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae01/12213063/086a67374f26/40_25001-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae01/12213063/03d559de64ac/40_25001-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae01/12213063/086a67374f26/40_25001-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae01/12213063/03d559de64ac/40_25001-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae01/12213063/086a67374f26/40_25001-g002.jpg

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本文引用的文献

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A novel Nitrospira lineage isolated from activated sludge using elevated temperatures.采用高温从活性污泥中分离出的新型硝化螺旋菌属。
FEMS Microbiol Lett. 2023 Jan 17;370. doi: 10.1093/femsle/fnad035.
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Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors.基于基因组的宏基因组学揭示绿弯菌门在厌氧氨氧化、活性污泥和产甲烷反应器中的作用。
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Effects of reducing, stabilizing, and antibiotic agents on "Candidatus Kuenenia stuttgartiensis".
减少、稳定和抗生素制剂对“Candidatus Kuenenia stuttgartiensis”的影响。
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Metagenomic Analysis of Five Phylogenetically Distant Anammox Bacterial Enrichment Cultures.基于宏基因组分析的 5 种进化上不同的厌氧氨氧化菌富集培养物。
Microbes Environ. 2022;37(3). doi: 10.1264/jsme2.ME22017.
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A 20-Year Journey of Partial Nitritation and Anammox (PN/A): from Sidestream toward Mainstream.20 年的部分亚硝化和厌氧氨氧化(PN/A)历程:从侧流到主流。
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MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants.MiDAS 4:用于研究污水处理厂中细菌群落的全长 16S rRNA 基因序列和分类学的全球目录。
Nat Commun. 2022 Apr 7;13(1):1908. doi: 10.1038/s41467-022-29438-7.
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Physiology of anammox adaptation to low temperatures and promising biomarkers: A review.厌氧氨氧化菌适应低温的生理学机制及潜在生物标志物研究进展:综述
Bioresour Technol. 2022 Apr;349:126847. doi: 10.1016/j.biortech.2022.126847. Epub 2022 Feb 12.
8
Zeimonas arvi gen. nov., sp. nov., of the family Burkholderiaceae, harboring biphenyl- and phenolic acid-metabolizing genes, isolated from a long-term ecological research field.泽莫纳氏菌属,新属,拜氏菌科,含有联苯和酚酸代谢基因,从一个长期生态研究领域中分离得到。
Antonie Van Leeuwenhoek. 2021 Dec;114(12):2101-2111. doi: 10.1007/s10482-021-01664-x. Epub 2021 Oct 2.
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DRAM for distilling microbial metabolism to automate the curation of microbiome function.利用动态随机存取存储器(DRAM)提取微生物代谢物以实现微生物组功能自动编目。
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Comparative Genome-Centric Analysis of Freshwater and Marine ANAMMOX Cultures Suggests Functional Redundancy in Nitrogen Removal Processes.淡水和海洋厌氧氨氧化培养物的比较基因组中心分析表明氮去除过程中存在功能冗余。
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