Yashima Nao, Minamizono Wataru, Matsunaga Hiroya, Lyu Jiazheng, Fujikawa Kaoru, Suito Hirai, Okunuki Takumi, Nakai Shingo, Ohsako Masafumi
Graduate School of Health and Sports Science, Toyo University, 1-7-11 Akabanedai, Kita-ku, Tokyo, 115-8650, Japan.
Graduate School of Human Life Design, Toyo University, 1-7-11 Akabanedai, Kita-ku, Tokyo, 115-8650, Japan.
J Bone Miner Metab. 2025 Apr 29. doi: 10.1007/s00774-025-01603-0.
We investigated the effects of non-contact electrical stimulation via a Vector-potential (VP) transformer, a novel physical therapy device, on bone healing in drill-hole injury models.
Six-week-old male Wistar rats, after a one-week acclimation period, were divided into three groups: the control group (CO), the bone injury group (BI), in which a drill-hole injury was created, and the VP stimulation group (VP), which received non-contact electrical stimulation via a VP transformer after bone injury. In the VP group, rats underwent stimulation at 200 kHz for 30 minutes per day, seven days per week.
The VP group exhibited increased bone formation as early as day 7 post-injury, with significantly higher bone volume than the BI group at all time points (day 7: p = 0.0003; day 14: p = 0.0024; day 21: p = 0.0001). By day 21, the VP group showed lighter toluidine blue staining and reduced biglycan immunoreactivity compared to the BI group. Bone mineral density also increased (p = 0.0008). Osteoblasts in the VP group displayed abundant cytoplasm and a high capacity for osteocalcin synthesis. Additionally, the VP group demonstrated increased expression of Bglap (day 5: p = 0.0068; day 7: p = 0.0096) and Ctsk (day 7: p = 0.0329; day 14: p = 0.0171), along with a higher number of TRAP-positive osteoclasts (day 21: p = 0.0159) compared to the BI group.
Non-contact electrical stimulation via a VP transformer promotes bone healing in drill-hole injury models.
我们研究了通过矢量电位（VP）变压器（一种新型物理治疗设备）进行的非接触式电刺激对钻孔损伤模型中骨愈合的影响。
六周龄雄性Wistar大鼠，经过一周的适应期后，分为三组：对照组（CO）、造成钻孔损伤的骨损伤组（BI），以及骨损伤后通过VP变压器接受非接触式电刺激的VP刺激组（VP）。在VP组中，大鼠每天以200kHz频率刺激30分钟，每周刺激七天。
VP组在损伤后第7天就显示出骨形成增加，在所有时间点（第7天：p = 0.0003；第14天：p = 0.0024；第21天：p = 0.0001）骨体积均显著高于BI组。到第21天，与BI组相比，VP组甲苯胺蓝染色更浅，双糖链蛋白聚糖免疫反应性降低。骨矿物质密度也增加（p = 0.0008）。VP组的成骨细胞显示出丰富的细胞质和较高的骨钙素合成能力。此外，与BI组相比，VP组Bglap的表达增加（第5天：p = 0.0068；第7天：p = 0.0096）和Ctsk的表达增加（第7天：p = 0.0329；第14天：p = 0.0171），并且抗酒石酸酸性磷酸酶阳性破骨细胞数量更多（第21天：p = 0.0159）。
通过VP变压器进行的非接触式电刺激可促进钻孔损伤模型中的骨愈合。
