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脂肪干细胞胶用于减轻全层皮肤移植收缩：一项小鼠实验研究

SVF-gel application for the alleviation of full-thickness skin graft contraction: an experimental study in mice.

作者信息

Ni Xuejun, Xu Nating, Shen Jian, Wan Xiaofen, Shen Shuyi, Lei Chen, Wang Songyu, Wang Biao, Shan Xiuying

机构信息

Department of Plastic Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.

Department of Plastic Surgery, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, China.

出版信息

Sci Rep. 2025 Apr 29;15(1):15082. doi: 10.1038/s41598-025-99649-7.

DOI:10.1038/s41598-025-99649-7
PMID:40301501
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12041231/
Abstract

Skin grafts often suffer from contracture, complicating recovery. SVF-gel shows promise in addressing scar contracture, but its therapeutic effects and mechanisms are not fully understood. This study evaluates the efficacy of SVF-gel in full-thickness skin grafting. Full-thickness skin grafts were harvested from mice dorsal skin, rotated and sutured. SVF-gel or saline was injected beneath the muscle fascia. Immunohistochemistry assessed SVF-gel's effects on angiogenesis, collagen deposition, fibrosis, and dermal adipocytes. Keloid-related genes from GSE92566 and GSE158395 were analyzed for functional enrichment and protein-protein interactions, with hub genes validated using GSE190626. SVF-gel significantly increased the grafted area, thickness of the epidermal and dermal adipose layers, and hair follicles compared to the control group. SVF-gel enhanced CD31 and perilipin expression, decreased α-SMA expression, and identified HLA cells around CD31 cells in the dermal microvessels and adipose tissue of the graft. Sixty commonly downregulated keloid-related genes were identified, with KEGG pathway analysis indicating enrichment in the PPAR signaling pathway and lipolysis regulation. Five hub genes (ADIPOQ, FABP4, KRT7, LEP, and PIP) were validated. SVF-gel shows promise as a stem cell therapy for skin grafts, improving outcomes by enhancing revascularization, increasing collagen fiber density and regularity, accelerating myofibroblast turnover, promoting adipogenesis, and increasing hair follicles.

摘要

皮肤移植常常会出现挛缩，使恢复过程变得复杂。脂肪来源干细胞凝胶（SVF-凝胶）在解决瘢痕挛缩方面显示出前景，但其治疗效果和机制尚未完全明确。本研究评估了SVF-凝胶在全层皮肤移植中的疗效。从小鼠背部皮肤获取全层皮肤移植片，进行旋转并缝合。在肌肉筋膜下注射SVF-凝胶或生理盐水。免疫组织化学评估了SVF-凝胶对血管生成、胶原蛋白沉积、纤维化和真皮脂肪细胞的影响。对来自GSE92566和GSE158395的瘢痕疙瘩相关基因进行功能富集和蛋白质-蛋白质相互作用分析，并使用GSE190626验证枢纽基因。与对照组相比，SVF-凝胶显著增加了移植面积、表皮和真皮脂肪层厚度以及毛囊数量。SVF-凝胶增强了CD31和脂滴蛋白的表达，降低了α-平滑肌肌动蛋白（α-SMA）的表达，并在移植皮肤的真皮微血管和脂肪组织中识别出CD31细胞周围的人类白细胞抗原（HLA）细胞。鉴定出60个常见的瘢痕疙瘩相关下调基因，KEGG通路分析表明其在过氧化物酶体增殖物激活受体（PPAR）信号通路和脂肪分解调节中富集。验证了5个枢纽基因（脂联素（ADIPOQ）、脂肪酸结合蛋白4（FABP4）、角蛋白7（KRT7）、瘦素（LEP）和磷脂酰肌醇蛋白聚糖（PIP））。SVF-凝胶作为一种用于皮肤移植的干细胞疗法显示出前景，通过增强血管再生、增加胶原纤维密度和规则性、加速肌成纤维细胞更新、促进脂肪生成以及增加毛囊数量来改善治疗效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9db1/12041231/0ed9e1ce1e88/41598_2025_99649_Fig8_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9db1/12041231/0ed9e1ce1e88/41598_2025_99649_Fig8_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9db1/12041231/73b267ffb9f1/41598_2025_99649_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9db1/12041231/0ed9e1ce1e88/41598_2025_99649_Fig8_HTML.jpg

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