Yamada Yuichi, Torii Akiko, Uruga Yukako, Sato Yuiko, Matsubara Yumiko, Matsumoto Morio, Nakamura Masaya, Sato Kazuki, Miyamoto Takeshi
Institute for Integrated Sports Medicine, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Sci Rep. 2025 Apr 29;15(1):15015. doi: 10.1038/s41598-025-99657-7.
Tendon and ligament disorders, such as tendinopathy, cause pain and limit levels of activities of daily living. Thus, devising methods to heal them is crucial. Although treatment with autologous platelet rich plasma (PRP) is reportedly useful against tendon injury, PRP requires blood sampling and its quality varies. Here we show that platelet-like cells (ASCL-PLCs) derived from a heterologous human adipose-derived mesenchymal stem cell line (ASCL) promote significant tendon repair in a collagenase-induced injury model in rat Achilles tendons. Single administration of human ASCL-PLCs to rat Achilles tendon after 2 weeks of collagenase treatment significantly increased tendon strength and improved semi-quantitative histological evaluation scores in 4 weeks relative to PBS-treated controls. Moreover, xeno-graft reactions were not evident in ASCL-PLC-administered rats. In vitro, ASCL-PLC treatment significantly upregulated Col1a1, Lox and Mkx gene expression in NIH3T3 fibroblasts and activated ERK signaling. Overall, ASCL-PLCs could serve as a useful tool to repair injured tendons and treat tendinopathy. This approach eliminates the need for blood sampling, ensures consistent quality, supports xeno-transplantation, and increases injured tendon strength.
肌腱和韧带疾病，如肌腱病，会引起疼痛并限制日常生活活动水平。因此，设计治愈它们的方法至关重要。尽管据报道自体富血小板血浆（PRP）治疗对肌腱损伤有用，但PRP需要采血且其质量存在差异。在此我们表明，源自异源人脂肪间充质干细胞系（ASCL）的类血小板细胞（ASCL-PLCs）在大鼠跟腱胶原酶诱导损伤模型中促进显著的肌腱修复。在胶原酶处理2周后对大鼠跟腱单次施用人ASCL-PLCs，相对于PBS处理的对照组，4周时显著增加了肌腱强度并改善了半定量组织学评估评分。此外，在施用ASCL-PLCs的大鼠中未明显出现异种移植反应。在体外，ASCL-PLC处理显著上调了NIH3T3成纤维细胞中Col1a1、Lox和Mkx基因的表达并激活了ERK信号传导。总体而言，ASCL-PLCs可作为修复损伤肌腱和治疗肌腱病的有用工具。这种方法无需采血，确保质量一致，支持异种移植，并增加损伤肌腱的强度。
