Stomatologic Hospital & Collage, Anhui Medical University, Hefei, 230032, China.
ACS Appl Mater Interfaces. 2012 Dec;4(12):6901-10. doi: 10.1021/am302041b. Epub 2012 Dec 4.
Inspired by mussel bioadhesion in nature, dopamine is extensively used for biomaterial surface modification. In this study, we coated dopamine on demineralized enamel and dentin surfaces to evaluate the effect of polydopamine coating on dental remineralization. Dental slices containing enamel and dentin were first etched with 37% phosphoric acid for 2 min, followed by immersion in a 2 mg/mL freshly prepared solution of dopamine (10 mM Tris buffer, pH 8.5) for approximately 24 h at room temperature in the dark to obtain polydopamine coating. Then, the dental slices with and without polydopamine coating were immersed in the supersaturated solution of calcium and phosphate at 37 °C for 2 and 7 days. The supersaturated solution of calcium and phosphate was refreshed each day. The precipitates were characterized by SEM, XRD, FTIR, microhardness, and nanoscratch analyses. No significant difference was observed in the remineralization of enamel whether it was coated with polydopamine or not. However, a significant difference was found in dentin remineralization between dentin with and without polydopamine coating. Polydopamine coating remarkably promoted demineralized dentin remineralization, and all dentin tubules were occluded by densely packed hydroxyapatite crystals. Thus, coating polydopamine on dental tissue surface may be a simple universal technique to induce enamel and dentin remineralization simultaneously.
受贻贝生物黏附作用的启发，多巴胺被广泛用于生物材料表面改性。在这项研究中，我们将多巴胺涂覆在脱矿牙釉质和牙本质表面，以评估聚多巴胺涂层对牙齿再矿化的影响。首先将含有牙釉质和牙本质的牙切片用 37%磷酸蚀刻 2 分钟，然后在室温黑暗条件下将其浸入 2 mg/mL 新制备的多巴胺溶液（10 mM Tris 缓冲液，pH 8.5）中约 24 小时，以获得聚多巴胺涂层。然后，将有和没有聚多巴胺涂层的牙切片在 37°C 的过饱和钙磷溶液中浸泡 2 天和 7 天。每天更换过饱和钙磷溶液。通过 SEM、XRD、FTIR、显微硬度和纳米划痕分析对沉淀物进行了表征。无论是否涂有聚多巴胺，牙釉质的再矿化都没有明显差异。然而，在有无聚多巴胺涂层的牙本质的再矿化之间发现了显著差异。聚多巴胺涂层显著促进了脱矿牙本质的再矿化，所有牙本质小管都被紧密堆积的羟基磷灰石晶体封闭。因此，在牙组织表面涂覆聚多巴胺可能是一种简单的通用技术，可以同时诱导牙釉质和牙本质再矿化。
