Fan Xin, Kannan Villalan Arivizhivendhan, Hu YeZhi, Wu XiaoDong, Wang HaoNing, Wang XiaoLong
College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, Heilongjiang Province, China.
Key Laboratory for Wildlife Diseases and Bio-Security Management of Heilongjiang Province, Harbin 150040, Heilongjiang Province, China.
Transbound Emerg Dis. 2024 Apr 9;2024:4374388. doi: 10.1155/2024/4374388. eCollection 2024.
(PPRV) causes a highly contagious and severe infectious disease known as (PPR), resulting in significant mortality in both domestic and wild ruminants. An in-depth understanding of the molecular relationship between PPRV and susceptible hosts is essential for the prevention of PPR. The signaling lymphocytic-activation molecule (SLAM) acts as a key receptor in susceptible host species, mediating interactions with PPRV and triggering PPR in ruminants. This study offers an in-depth analysis of PPRV-susceptible host species as well as the identified SLAM amino acid sequences to date. Investigation reveals that nine families-, , , , , , , , and -have been affected by PPRV infection. Furthermore, a bioinformatics-based approach was proposed to screen the least common amino acid patterns (LCAP) in important SLAM receptor regions of known PPRV-susceptible species. Research findings reveal that 14 least common amino acid sites (LCAS) in SLAM amino acid sequences (I61, I63, S60, S70, K76, K78, I79, S81, L82, E123, N125, S127, V128, and F131) exhibit a prevalent similarity to LCAP across all known susceptible species. Comparative analysis of these 14 LCAP with SLAM nucleotide sequences from unknown susceptible ruminants to identify species at heightened risk of PPRV. In the result, 48 species from 20 different families across six orders were at potential risk of being infected with PPRV. This exploration suggests the feasibility of assessing potential hosts at high risk of PPRV infection through the LCAS screening technique. Moreover, it offers a means to anticipate and issue warnings regarding the likelihood of interspecies transmission. In conclusion, this study integrates molecular biology and bioinformatics, shedding light on PPRV infection dynamics and paving the way for predictive strategies to prevent the spread of this devastating disease among ruminant populations.
小反刍兽疫病毒（PPRV）会引发一种极具传染性的严重传染病，即小反刍兽疫（PPR），导致家养和野生反刍动物大量死亡。深入了解PPRV与易感宿主之间的分子关系对于预防PPR至关重要。信号淋巴细胞激活分子（SLAM）在易感宿主物种中充当关键受体，介导与PPRV的相互作用并引发反刍动物的PPR。本研究对PPRV易感宿主物种以及迄今为止已鉴定的SLAM氨基酸序列进行了深入分析。调查显示，有九个科——、、、、、、、、和——受到了PPRV感染的影响。此外，还提出了一种基于生物信息学的方法，以筛选已知PPRV易感物种重要SLAM受体区域中最不常见的氨基酸模式（LCAP）。研究结果表明，SLAM氨基酸序列中的14个最不常见氨基酸位点（LCAS）（I61、I63、S60、S70、K76、K78、I79、S81、L82、E123、N125、S127、V128和F131）在所有已知易感物种中与LCAP表现出普遍相似性。对这14个LCAP与未知易感反刍动物的SLAM核苷酸序列进行比较分析，以识别PPRV感染风险较高的物种。结果显示，六个目20个不同科的48个物种有感染PPRV的潜在风险。这一探索表明，通过LCAS筛选技术评估PPRV感染高风险潜在宿主具有可行性。此外，它还提供了一种预测和发布关于种间传播可能性警告的方法。总之，本研究将分子生物学和生物信息学相结合，阐明了PPRV感染动态，为预防这种毁灭性疾病在反刍动物群体中传播的预测策略铺平了道路。
