Chaiyawong Supassama, Charoenkul Kamonpan, Jairak Waleemas, Udom Kitikhun, Chamsai Ekkapat, Techakriengkrai Navapon, Suwannakarn Kamol, Amonsin Alongkorn
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Emerging and Re-Emerging Infectious Diseases in Animals, Center of Excellence, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Transbound Emerg Dis. 2024 Feb 20;2024:9938523. doi: 10.1155/2024/9938523. eCollection 2024.
SARS-CoV-2 causes the coronavirus disease 2019 (COVID-19) pandemic. Cross-species transmission of SARS-CoV-2 from humans to domestic animals has been reported. In this study, we conducted a serological survey and molecular investigation of SARS-CoV-2 infection in domestic dogs and cats in Bangkok and the vicinities from January 2021 to August 2022. A total of 2,664 serum samples were examined for antibodies against SARS-CoV-2 using nucleocapsid protein-based ELISA (NP-ELISA). Our result showed 2.28% (33/1,446) seropositivity in dogs and 1.81% (22/1,218) in cats. The positive NP-ELISA serum samples were confirmed using a surrogate virus neutralization test (sVNT). Of 55 seropositive samples by NP-ELISA, two dogs and 19 cats were confirmed seropositive by sVNT. Our result supported the serological evidence of SARS-CoV-2 exposure in domestic dogs and cats. We also investigated SARS-CoV-2 infection by real-time RT-PCR in 156 domestic dogs and cats in COVID-19-positive households. Our result showed active SARS-CoV-2 infection in a dog living with COVID-19 positive owner. Genetic and phylogenetic analysis of the SARS-CoV-2 from the dog and its owner confirmed the SARS-CoV-2 variant Omicron BA.2. It is the first report of SARS-CoV-2 Omicron variant in pet living in COVID-19-positive household in Thailand.
严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）引发了2019冠状病毒病（COVID-19）大流行。有报告称SARS-CoV-2已从人类跨物种传播到家养动物。在本研究中，我们于2021年1月至2022年8月期间，对曼谷及其周边地区的家犬和家猫进行了SARS-CoV-2感染的血清学调查和分子研究。使用基于核衣壳蛋白的酶联免疫吸附测定（NP-ELISA）对总共2664份血清样本进行了SARS-CoV-2抗体检测。我们的结果显示，犬类血清阳性率为2.28%（33/1446），猫类为1.81%（22/1218）。使用替代病毒中和试验（sVNT）对NP-ELISA检测呈阳性的血清样本进行了确认。在55份NP-ELISA血清阳性样本中，有2只犬和19只猫经sVNT确认为血清阳性。我们的结果支持了家犬和家猫接触过SARS-CoV-2的血清学证据。我们还对156只来自COVID-19阳性家庭的家犬和家猫进行了实时逆转录聚合酶链反应（RT-PCR）以调查SARS-CoV-2感染情况。我们的结果显示，有一只与COVID-19阳性主人共同生活的犬感染了活跃的SARS-CoV-2。对该犬及其主人身上的SARS-CoV-2进行的基因和系统发育分析证实为SARS-CoV-2奥密克戎BA.2变体。这是泰国首次报告生活在COVID-19阳性家庭中的宠物感染SARS-CoV-2奥密克戎变体。
