Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Transbound Emerg Dis. 2022 Jul;69(4):2140-2147. doi: 10.1111/tbed.14208. Epub 2021 Jul 8.
Coronavirus disease of 2019 (COVID-19) caused by severe acute respiratory syndrome virus type 2 (SARS-CoV-2) is an emerging severe acute respiratory disease affecting global human health. In this study, a large-scale serological survey of antibodies against SARS-CoV-2 in dogs and cats was conducted during the first and second waves of COVID-19 outbreaks in Thailand, from April to December 2020. A total of 3215 serum samples were collected from dogs (n = 2102) and cats (n = 1113) living in Bangkok and in the vicinities. Serum samples were tested for SARS-CoV-2 antibodies by using an indirect multispecies enzyme-linked immunosorbent assay (ELISA). Positive and suspected samples were additionally tested for neutralizing antibodies by the surrogate virus neutralization test (sVNT). The indirect ELISA results showed that 1.66% (35 out of 2103) of dogs and 0.36% (four out of 1112) of cats were positive for SARS-CoV-2 antibodies. The sVNT results showed that all ELISA-positive and suspected samples were negative for neutralizing antibodies. Positive serum samples (35 dogs and four cats) were obtained from clinically healthy animals and animals with mild respiratory signs aged <1-13 years living in Bangkok and Samutprakarn Provinces. In summary, a serological survey revealed evidence of anti-N-IgG antibodies suggesting SARS-CoV-2 exposure in both dogs and cats during the first and second COVID-19 outbreaks in Thailand.
2019 年冠状病毒病（COVID-19）是由严重急性呼吸系统综合症冠状病毒 2 型（SARS-CoV-2）引起的一种新兴的严重急性呼吸道疾病，影响着全球人类健康。在这项研究中，我们在泰国 COVID-19 爆发的第一波和第二波期间，对犬和猫体内针对 SARS-CoV-2 的抗体进行了大规模的血清学调查，时间为 2020 年 4 月至 12 月。我们从曼谷及其周边地区的 2102 只犬和 1113 只猫中采集了 3215 份血清样本。我们使用间接多物种酶联免疫吸附测定（ELISA）法检测血清样本中的 SARS-CoV-2 抗体。对阳性和疑似样本，我们用替代病毒中和试验（sVNT）进一步检测中和抗体。间接 ELISA 结果显示，1.66%（2103 只犬中的 35 只）的犬和 0.36%（1112 只猫中的 4 只）的猫 SARS-CoV-2 抗体检测结果呈阳性。sVNT 结果显示，所有 ELISA 阳性和疑似样本的中和抗体检测结果均为阴性。阳性血清样本（35 只犬和 4 只猫）来自曼谷和 Samutprakarn 府的无临床症状或有轻微呼吸道症状的<1-13 岁健康动物和患病动物。综上所述，血清学调查结果表明，在泰国 COVID-19 的第一波和第二波爆发期间，犬和猫体内均存在针对 SARS-CoV-2 的 N-IgG 抗体，提示曾暴露于 SARS-CoV-2。
