放疗后白化大鼠下颌下腺的长期再生潜力：细胞角蛋白17重新分布的作用

Long-Term Regenerative Potential of Submandibular Glands in Albino Rats Following Radiotherapy: Role of Cytokeratin 17 Redistribution.

作者信息

Hassan Sherif S, Alqahtani Mashael

机构信息

Department of Oral Biology, Faculty of Dentistry, Al Azhar University, Cairo, EGY.

Department of Basic and Clinical Oral Sciences, College of Dental Medicine, Umm Al-Qura University, Makkah, SAU.

出版信息

Cureus. 2025 Mar 30;17(3):e81465. doi: 10.7759/cureus.81465. eCollection 2025 Mar.

DOI:10.7759/cureus.81465

PMID:40303535

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12040290/

Abstract

BACKGROUND

Fractionated radiotherapy (RT) is a common approach for treating head and neck malignancies in which the salivary glands become included within the radiation field, causing xerostomia. This study aimed to evaluate the long-term effects of post-dose RT to determine the possibility of recovery of the submandibular gland (SMG) without intervention or further deterioration.

METHODS

Twenty-seven albino rats were divided into three equal groups, each containing nine rats. The first group is the control group, which was not exposed to radiation. The second and third groups served as the experimental groups, receiving a total radiation dose of 30 Gy, divided into 5 Gy doses per day over six consecutive days. The SMGs were extracted and prepared for histopathological and immunohistochemical analysis, one month after irradiation for Group II and one year after irradiation for Group III.

RESULTS

Immunohistochemical analysis of the control salivary gland revealed weak to mild staining for cytokeratin 17 (CK17) (intermediate filaments) in the cells of the duct system and serous acinar cells. The irradiated SMG exhibited significantly moderate to strong cytokeratin expressions in the duct cells (P=0.000 for Group II and P=0.025 for Group III) compared with the control. In contrast, the serous acinar cells in Group II exhibited significantly increased immunostaining for cellular keratin (P = 0.001) compared to the control group. Meanwhile, the acinar cells in Group III showed CK17 expression levels similar to those observed in the control group (P = 0.297).

CONCLUSION

CK17 expression differed significantly between the control and the second group, which underwent a one-month wait after the final radiation dose. In contrast, the third group, which underwent a one-year wait, showed intermediate results between the two groups, indicating a more advanced stage of recovery. Statistical analysis showed differing recovery dynamics, with ductal cells exhibiting slower recovery than acinar cells, which appeared more advanced in their healing. These findings highlight the progressive yet varied repair processes within the irradiated glands.

摘要

背景

分割放射治疗（RT）是治疗头颈部恶性肿瘤的常用方法，在此过程中唾液腺会被纳入辐射野，从而导致口干症。本研究旨在评估放疗后长期影响，以确定下颌下腺（SMG）在无干预情况下恢复或进一步恶化的可能性。

方法

将27只白化大鼠平均分为三组，每组9只。第一组为对照组，未接受辐射。第二组和第三组为实验组，接受总剂量30 Gy的辐射，连续6天每天分剂量5 Gy照射。照射后1个月对第二组、照射后1年对第三组的下颌下腺进行提取并准备进行组织病理学和免疫组织化学分析。

结果

对照唾液腺的免疫组织化学分析显示，导管系统细胞和浆液性腺泡细胞中细胞角蛋白17（CK17）（中间丝）呈弱阳性至轻度染色。与对照组相比，照射后的下颌下腺导管细胞中细胞角蛋白表达明显为中度至强阳性（第二组P = 0.000，第三组P = 0.025）。相比之下，与对照组相比，第二组浆液性腺泡细胞的细胞角蛋白免疫染色显著增加（P = 0.001）。同时，第三组腺泡细胞的CK17表达水平与对照组相似（P = 0.297）。