Apple F S, Rogers M A, Casal D C, Sherman W M, Ivy J L
J Appl Physiol (1985). 1985 Jul;59(1):149-53. doi: 10.1152/jappl.1985.59.1.149.
The creatine kinase (CK) isoenzyme composition was determined in serial gastrocnemius muscle biopsies obtained from 12 male marathon runners. The mean muscle CK-MB composition significantly increased after chronic exercise (training) from 5.3% (pretraining) to 7.7% (premarathon) as well as after acute exercise (postmarathon) to 10.5% of the total CK activity (P less than 0.05). However, no significant differences in total CK activities were detected. Additionally, mitochondrial CK and CK-BB isoenzymes were present in muscle homogenates. A significant correlation was observed in the increase in mean serum total CK (3,322 U/l) and CK-MB (174 U/l) activities 24 h after the race (r = 0.98, P less than 0.05). These results show that gastrocnemius muscle adapts to long-distance training and racing with increased CK-MB activities and imply that skeletal muscle is the major source of elevated serum CK-MB activities in marathon runners.
对12名男性马拉松运动员的腓肠肌进行系列活检，测定肌酸激酶（CK）同工酶组成。长期运动（训练）后，平均肌肉CK-MB组成从训练前的5.3%显著增加到马拉松赛前的7.7%，急性运动（马拉松赛后）后增加到总CK活性的10.5%（P<0.05）。然而，未检测到总CK活性有显著差异。此外，肌肉匀浆中存在线粒体CK和CK-BB同工酶。赛后24小时，平均血清总CK（3322 U/l）和CK-MB（174 U/l）活性的增加之间存在显著相关性（r=0.98，P<0.05）。这些结果表明，腓肠肌通过增加CK-MB活性来适应长距离训练和比赛，这意味着骨骼肌是马拉松运动员血清CK-MB活性升高的主要来源。
