Ninkuu Vincent, Liu Zhixin, Liu Hao, Li Chunyang, Zhou Yaping, Zhao Qianli, Qin Aizhi, Li Mengfan, Gao Peibo, Yan Lulu, Song Xiao, Kong Luyao, Xie Yajie, Guo Enzhi, Sun Xuwu
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, 475001, China.
Sci Rep. 2025 Apr 30;15(1):15143. doi: 10.1038/s41598-025-99279-z.
Verticillium dahliae is a soilborne pathogenic fungus that causes vascular discoloration and wilting in a broad spectrum of plant hosts, affecting about 400 species, such as cotton, potatoes, watermelon, cucumber, spinach, etc. In 2021, V. dahliae was estimated to cause about 15-20% reduction in cotton in China. Here, we report the genome sequencing of a novel strain named huangweibingjun, isolated from diseased cotton roots in the Henan province of China. The huangweibingjun genome consists of a total size of 35.84 Mb, GC content of 59.835%, and harbors six chromosomes (scaffold7561, scaffold7329, scaffold7795, scaffold5491, scaffold5473, and scaffold4511). The genome architecture showed a high diversity of cell wall-degrading secretory proteins that might influence the pathogenicity of the fungal strain. Moreover, preliminary metabolic pathway prediction showed that this novel strain synthesizes polyketide, terpenoids, shikimic acid-derived compounds and could also be aflatoxigenic. Consistent with other pathogenic microbes, the huangweibingjun genome comprises several virulent-associated genes. This genome assembly lays the foundation for further investigation of the pathogenicity of huangweibingjun.
大丽轮枝菌是一种土传致病真菌，可导致多种植物宿主出现维管束变色和枯萎，影响约400种植物，如棉花、马铃薯、西瓜、黄瓜、菠菜等。2021年，据估计大丽轮枝菌在中国导致棉花减产约15%-20%。在此，我们报告了从中国河南省患病棉花根部分离出的一种名为“黄萎病菌”的新菌株的基因组测序结果。黄萎病菌基因组总大小为35.84 Mb，GC含量为59.835%，包含六条染色体（scaffold7561、scaffold7329、scaffold7795、scaffold5491、scaffold5473和scaffold4511）。基因组结构显示细胞壁降解分泌蛋白具有高度多样性，这可能影响该真菌菌株的致病性。此外，初步代谢途径预测表明，这种新菌株可合成聚酮化合物、萜类化合物、莽草酸衍生化合物，并且可能产生黄曲霉毒素。与其他致病微生物一致，黄萎病菌基因组包含多个与毒性相关的基因。该基因组组装为进一步研究黄萎病菌的致病性奠定了基础。
