一种用于三维腔状心脏类器官多能干细胞建模的稳健方案。
A Robust Protocol for Pluripotent Stem Cell Modeling of 3D Chamber-Like Cardiac Organoids.
作者信息
Pucéat Michel
机构信息
INSERM U1263, INRAE U1260 C2VN Cardiovascular and Nutrition Center, Aix-Marseille University, Marseille, France.
出版信息
Methods Mol Biol. 2025;2924:93-100. doi: 10.1007/978-1-0716-4530-7_7.
PMID:40307637
Abstract
The formation of cardiac organoids brings a new 3D model of cardiac development. The organoid is also very helpful in screening drug efficiency or toxicity. Inspired by cardiac biology of development, a step-by-step protocol is described to generate cardiac chamber-like organoids from pluripotent stem cells. A few examples of functional tests and the use of these organoids for drug toxicity are reported.
摘要
心脏类器官的形成带来了心脏发育的全新三维模型。该类器官在筛选药物效率或毒性方面也非常有帮助。受心脏发育生物学启发，本文描述了一种从多能干细胞生成类心脏腔室器官的逐步方案。文中还报道了一些功能测试的实例以及这些类器官在药物毒性研究中的应用。
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