de Capoa A, Marlekaj P, Baldini A, Archidiacono N, Rocchi M
J Cell Sci. 1985 Mar;74:21-35. doi: 10.1242/jcs.74.1.21.
The activity of ribosomal gene clusters has been studied by cytological methods in human cultured cells grown in different amounts of serum under controlled experimental conditions. It has been shown that increasing amounts of serum induce an increase in ribosomal RNA synthesis at the single cell level. Furthermore, the concomitant identification of individual rRNA gene clusters by fluorescent techniques allowed us to demonstrate: (1) that individual gene clusters have differential transcriptional activity and differential frequency of activation; (2) that ribosomal gene activity is closely associated with the amount of silver-positive gene product and; (3) that environmental variations modulate rRNA synthesis by repressing or derepressing specific gene clusters.
在可控实验条件下，利用细胞学方法对在不同血清量中生长的人类培养细胞的核糖体基因簇活性进行了研究。结果表明，血清量增加会在单细胞水平上诱导核糖体RNA合成增加。此外，通过荧光技术对单个rRNA基因簇进行同步鉴定，使我们能够证明：（1）单个基因簇具有不同的转录活性和不同的激活频率；（2）核糖体基因活性与银阳性基因产物的量密切相关；（3）环境变化通过抑制或解除对特定基因簇的抑制来调节rRNA合成。
