Everaerts F M, Verheggen T P, Reijenga J C, Aben G V, Gebauer P, Bocek P
J Chromatogr. 1985 Feb 22;320(1):263-8. doi: 10.1016/s0021-9673(01)90504-7.
The suitability of isotachophoresis for the analysis of metals in, e.g., environmental samples was studied. In a cationic operational system the heavy metals Fe, Cu, Ni, Cd, Co, Zn, Pb and Mn were simultaneously determined. The separation was achieved through complex formation with one of the counter ions, hydroxyisobutyric acid. The other counter ion was acetic acid, the leading ion was 0.02 M potassium or sodium (pH 4.1) and the terminator was H+. The analysis time was 15 min at 60 microA in a 0.2 mm I.D. capillary. Aqueous samples containing ppm and ppb amounts were enriched on a cation exchanger with an extremely low affinity for sodium (Chelex 100). Good recovery, linearity, precision and accuracy were obtained even down to the ppb range. Although the sensitivity of the method is not greater than that of some of the more established methods for the individual metals, a great advantage of isotachophoresis is the simultaneous determination of the metals, with equal response factors. An example is given of the determination of metals, including aluminium, in serum.
研究了等速电泳用于分析例如环境样品中金属的适用性。在阳离子操作体系中，同时测定了重金属铁、铜、镍、镉、钴、锌、铅和锰。通过与一种抗衡离子羟基异丁酸形成络合物实现分离。另一种抗衡离子是乙酸，先导离子是0.02 M钾或钠（pH 4.1），终止离子是H⁺。在内径0.2 mm的毛细管中，60 μA下分析时间为15分钟。含有ppm和ppb含量的水样在对钠亲和力极低的阳离子交换剂（螯合树脂100）上进行富集。即使在ppb范围内也能获得良好的回收率、线性、精密度和准确度。尽管该方法的灵敏度不高于某些针对个别金属的更成熟方法，但等速电泳的一个很大优势是能同时测定金属，且响应因子相同。给出了血清中包括铝在内的金属测定示例。
