Kuhn Madeline R, Wolcott Emma A, Langer Ellen M
Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, OR, United States.
Division of Oncological Sciences, Oregon Health and Science University, Portland, OR, United States.
Front Bioeng Biotechnol. 2025 Apr 17;13:1521044. doi: 10.3389/fbioe.2025.1521044. eCollection 2025.
Culture platforms that closely mimic the spatial architecture, cellular diversity, and extracellular matrix composition of native tissues can serve as invaluable tools for a range of scientific discovery and biomedical applications. Organoids have emerged as a promising alternative to both traditional 2D cell culture and animal models, offering a physiologically relevant 3D culture system for studying human cell biology. Organoids provide a manipulable platform to investigate organ development and function as well as to model patient-specific phenotypes. This mini review examines various methods used for culturing organoids to model normal and disease conditions in gastrointestinal tissues. We focus on how the matrix composition and media formulations can impact cell signaling, altering the baseline cellular phenotypes as well as response to perturbations. We discuss future directions for optimizing organoid culture conditions to improve basic and translational potential.
能够紧密模拟天然组织的空间结构、细胞多样性和细胞外基质组成的培养平台，可作为一系列科学发现和生物医学应用的宝贵工具。类器官已成为传统二维细胞培养和动物模型的一种有前景的替代方案，为研究人类细胞生物学提供了一个生理相关的三维培养系统。类器官提供了一个可操作的平台，用于研究器官发育和功能以及模拟患者特异性表型。这篇小型综述探讨了用于培养类器官以模拟胃肠道组织正常和疾病状态的各种方法。我们关注基质组成和培养基配方如何影响细胞信号传导，改变基线细胞表型以及对干扰的反应。我们讨论了优化类器官培养条件以提高基础和转化潜力的未来方向。
