Wang Juan, Su Xinru, Jia Zhicheng, Peng Wenxin, Dou Liru, Mao Peisheng
College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China.
BMC Plant Biol. 2025 May 2;25(1):575. doi: 10.1186/s12870-025-06616-5.
Alfalfa is widely regarded as one of the most important forage crops globally. However, its growth and development are primarily constrained by various abiotic stresses. FIMBRINs are crucial actin-binding proteins involved in regulating cellular dynamics in plants under various stress conditions and during developmental processes. The Fimbrin (FIM) gene family has been reported only in Arabidopsis, while a comprehensive identification of the FIM gene family in alfalfa and the responses of its members to abiotic stresses remain unclear.
In this study, six MsFIM genes were identified in the alfalfa genome, distributed across three chromosomes. Phylogenetic analysis grouped these genes into four clades, all containing the conserved CH domain. Gene duplication events suggested that large fragment duplications contribute to gene amplification. Furthermore, cis-regulatory element analysis highlighted their pivotal roles in plant development and response to external abiotic stresses. RT-qPCR analyses revealed that the MsFIM genes exhibited differential expression across various tissues, with predominant expression in flowers, stems, and leaves. The MsFIM genes showed elevated expression under abiotic stresses (drought, cold, and salt) as well as hormone treatment (abscisic acid, ABA), suggesting that they served as positive regulators in alfalfa's resistance to abiotic stresses and its growth and development.
This study investigates the MsFIM genes in alfalfa, further analyzing their potential roles in plant development and response to abiotic stresses. These findings will provide novel insights into the molecular mechanisms of alfalfa's stress response.
紫花苜蓿被广泛认为是全球最重要的饲料作物之一。然而，其生长发育主要受到各种非生物胁迫的限制。肌动蛋白结合蛋白（FIMBRINs）是关键的肌动蛋白结合蛋白，参与调节植物在各种胁迫条件下和发育过程中的细胞动态。目前仅在拟南芥中报道了肌动蛋白结合蛋白（FIM）基因家族，而对紫花苜蓿中FIM基因家族的全面鉴定及其成员对非生物胁迫的响应仍不清楚。
在本研究中，在紫花苜蓿基因组中鉴定出6个MsFIM基因，分布在3条染色体上。系统发育分析将这些基因分为4个进化枝，所有进化枝都含有保守的CH结构域。基因复制事件表明大片段复制有助于基因扩增。此外，顺式调控元件分析突出了它们在植物发育和对外部非生物胁迫响应中的关键作用。RT-qPCR分析表明，MsFIM基因在不同组织中表现出差异表达，在花、茎和叶中表达占主导。MsFIM基因在非生物胁迫（干旱、寒冷和盐胁迫）以及激素处理（脱落酸，ABA）下表达升高，表明它们在紫花苜蓿对非生物胁迫的抗性及其生长发育中作为正向调节因子发挥作用。
本研究对紫花苜蓿中的MsFIM基因进行了研究，进一步分析了它们在植物发育和对非生物胁迫响应中的潜在作用。这些发现将为紫花苜蓿胁迫响应的分子机制提供新的见解。
