Yin Guoxing, Yu Ting, Lian Chunhua, Li Yang, Liu Dian, Li Haitao, Zhou Huijun, Yin Peng, Yao Shouzhuo
Department of Gastroenterology and Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, 410013, P. R. China.
Institute of Interdisciplinary Studies, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, P. R. China.
Adv Healthc Mater. 2025 Jun;14(16):e2404993. doi: 10.1002/adhm.202404993. Epub 2025 May 3.
The ability to perform simultaneous fluorescence imaging of multiple targets is essential, providing crucial multi-parametric information necessary for understanding complex biological interactions and processes. In this study, TBC, a novel multi-signal fluorescent probe is presented, crafted for simultaneous differentiation and in situ real-time monitoring of homocysteine (Hcy), cysteine (Cys), sulfur dioxide (SO), and glutathione (GSH), illuminating the dynamic metabolic status of endogenous reactive sulfur species. TBC achieves an ultrahigh signal-to-background ratio, enabling wash-free direct fluorescence imaging of the dynamics and distribution of these entities in living cells and zebrafish. Notably, TBC has revealed distinctive dynamic metabolic features of Hcy/Cys/SO/GSH during apoptosis and ferroptosis. This innovative probe acts as a key tool for unraveling the conversion networks of multiple reactive sulfur species and assessing the impact of metabolic oscillations during programmed cell death and the progression of diverse diseases, effectively uncovering concurrent biochemical dynamics in various biological settings and cell death events.
能够对多个靶点进行同步荧光成像至关重要，它能提供理解复杂生物相互作用和过程所需的关键多参数信息。在本研究中，提出了一种新型多信号荧光探针TBC，它用于同型半胱氨酸（Hcy）、半胱氨酸（Cys）、二氧化硫（SO）和谷胱甘肽（GSH）的同时区分和原位实时监测，揭示内源性活性硫物质的动态代谢状态。TBC实现了超高的信背比，能够对这些物质在活细胞和斑马鱼中的动态和分布进行免洗直接荧光成像。值得注意的是，TBC揭示了凋亡和铁死亡过程中Hcy/Cys/SO/GSH独特的动态代谢特征。这种创新探针是解开多种活性硫物质转化网络以及评估程序性细胞死亡和各种疾病进展过程中代谢振荡影响的关键工具，有效地揭示了各种生物环境和细胞死亡事件中的并发生化动态。
