Alsawaf K, Tu A T
J Forensic Sci. 1985 Jul;30(3):922-30.
Isotachophoresis, a technique to separate components by constant current electrophoresis, was used to differentiate between bloodstains of male, female, menstrual, bovine, and ovine bloods on cotton cloth and filter paper. Bloodstain analysis by isotachophoresis of stains from male and female subjects showed identical cationic patterns, but gave different profiles in the anionic system. Plasma had one extra peak in the anionic system when compared to the profile of serum. This extra peak is due to the presence of fibrinogen in plasma. Some hemoglobin peaks overlapped with serum protein peaks, but these could be identified by comparisons at lower concentrations. Menstrual blood had a much different pattern than normal human blood as was expected since many more compounds are found in menstrual blood than in normally circulating blood. Human, bovine, and ovine bloodstains showed different profiles both in the cationic and anionic systems. These results indicate that isotachophoresis can be used for the rapid and simple analysis of bloodstains to differentiate reliably human male, female, and menstrual blood and also to distinguish human bloodstains from those of cattle or sheep.
等速电泳是一种通过恒流电泳分离成分的技术，用于区分棉布和滤纸上男性、女性、月经血、牛血和羊血的血迹。对男性和女性受试者血迹进行等速电泳分析显示，阳离子模式相同，但在阴离子系统中呈现不同的图谱。与血清图谱相比，血浆在阴离子系统中有一个额外的峰。这个额外的峰是由于血浆中存在纤维蛋白原。一些血红蛋白峰与血清蛋白峰重叠，但可以通过较低浓度下的比较来识别。月经血的图谱与正常人类血液有很大不同，这是预期的，因为月经血中发现的化合物比正常循环血液中多得多。人类、牛和羊的血迹在阳离子和阴离子系统中都显示出不同的图谱。这些结果表明，等速电泳可用于血迹的快速简单分析，以可靠地区分人类男性、女性和月经血，还能将人类血迹与牛或羊的血迹区分开来。
