Mao Xufeng, Wang Yulong, Zhang Xiqian, Yao Zheyu, Yuan Zheyang, Yao Liwei, Wang Leining, Mao Haijiao
Department of Orthopaedic Surgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, People's Republic of China.
Department of Hand and foot surgery, the People's Hospital of Beilun District, Ningbo, 315800, Zhejiang, China.
Sci Rep. 2025 May 3;15(1):15556. doi: 10.1038/s41598-025-00596-0.
Adhesions and poor healing are major complications after Achilles tendon injury, and there is no effective solution to this problem. The purpose of this study is to determine whether the biomimetic peritoneurosis can solve the above problems in the process of Achilles tendon healing; This study investigated the adhesion and proliferation of tendon-derived stem cells (TDSCs) on dAM in vitro, as well as their tenogenic differentiation. The effects of Achilles tendon rupture on tendon regeneration were assessed in vivo by using an Achilles tendon rupture model in rats; Finally, from in vitro mRNA transcriptome sequencing combined with in vivo Achilles tendon protein to omics analysis to explore the repair mechanism of Achilles tendon rupture. Student's t-tests were used to assess the significance of observed differences between the two experimental groups. Multiple groups were compared using one-way analysis of variances (ANOVAs), followed by post hoc Bonferroni comparisons. The main findings of this study are that cell adhesion, proliferation, and differentiation of TDSCs were enhanced by dAM. Implanted dAM + TDSCs significantly accelerated tendon regeneration in vivo. In addition, extracellular matrix-related differential genes and proteins were screened by mRNA transcriptometry in vitro and proteomic analysis of Achilles tendon in vivo, and ERK signaling pathway was further explored to participate in the repair of Achilles tendon rupture. The dAM-TDSCs composite biomimetic peritendinous membrane material can effectively promote the healing of Achilles tendon. It provides a new direction for the development of biomimetic peritendinous membrane materials.
粘连和愈合不良是跟腱损伤后的主要并发症，且该问题尚无有效解决方案。本研究的目的是确定仿生腱周膜能否在跟腱愈合过程中解决上述问题；本研究调查了肌腱来源干细胞（TDSCs）在脱细胞羊膜（dAM）上的体外黏附、增殖及其成腱分化情况。通过大鼠跟腱断裂模型在体内评估跟腱断裂对肌腱再生的影响；最后，从体外mRNA转录组测序结合体内跟腱蛋白质组学分析来探究跟腱断裂的修复机制。采用Student's t检验评估两个实验组之间观察到的差异的显著性。多组比较采用单因素方差分析（ANOVA），随后进行事后Bonferroni比较。本研究的主要发现是，dAM可增强TDSCs的细胞黏附、增殖和分化。植入dAM + TDSCs可显著加速体内肌腱再生。此外，通过体外mRNA转录组学和体内跟腱蛋白质组学分析筛选出细胞外基质相关差异基因和蛋白质，并进一步探究ERK信号通路参与跟腱断裂的修复。dAM-TDSCs复合仿生腱周膜材料可有效促进跟腱愈合。它为仿生腱周膜材料的开发提供了新方向。
