Shri Manju, Pandey Vibha
School of Applied Sciences and Technology, Gujarat Technological University, Chandkheda, Ahmedabad, Gujarat, India.
Biotechnology and Molecular Biology Division, CSIR-National Botanical Research Institute, Lucknow, India.
Sci Rep. 2025 May 3;15(1):15530. doi: 10.1038/s41598-025-98130-9.
Current study described an efficient direct and indirect plantlet regeneration using nodal and leaf explants. The use of BAP alone promoted direct regeneration from nodal as well as leaf explants. Together with kinetin and IAA, BAP resulted in 5-7 regenerated shoots. Upon increasing the concentration BAP (3 mg/l) together with IAA (0.5 mg/l) and myoinositol (100 mg/l) explants produced a maximum of 6 ± 2 and 11 ± 3 regenerated shoots from leaf and nodal explants in 15 days, respectively. Continuous culture of explants of kinetin-supplemented media supported in vitro flowering. Flowers were smaller in size without seed setting. Supplementation of NAA along with cytokinin supported callus formation. However, NAA alone was very advantageous for in vitro rooting. The developed plantlets were transferred to the soil. The seed germination percentage was improved after a small incision opposite the micropylar end. A small cut helped in the inspection of the seed quality and also improved seed germination from 2 to 93%. GA (2 or 5 mg/l) does not influence breaking the seed dormancy. Just after the radical emergence, these seeds were transferred to MS media supplemented with elicitors (salicylic acid or jasmonic acid). Growth parameters of the seedlings were recorded from 6 to 18 of treatment. Though elicitors reduced the growth of seedlings, ascorbate peroxidase activity was enhanced by 10-14-fold as the concentration of SA/JA increased. Elicitors are known to enhance secondary metabolite (psoralen) in plants, and the threefold elevated expression of the psoralen synthase gene also proved the same. The study successfully established an efficient regeneration protocol for Psoralea corylifolia through direct and indirect organogenesis, significantly enhancing shoot regeneration, seed germination, and in vitro flowering. Additionally, elicitor treatments not only induced stress responses but also promoted higher psoralen accumulation, making this approach promising for large-scale propagation and secondary metabolite production.
当前的研究描述了一种利用节段和叶片外植体进行高效直接和间接植株再生的方法。单独使用BAP可促进节段和叶片外植体的直接再生。BAP与激动素和吲哚乙酸一起使用时，可产生5 - 7个再生芽。当将BAP浓度增加到3mg/l并与吲哚乙酸（0.5mg/l）和肌醇（100mg/l）一起使用时，外植体在15天内分别从叶片和节段外植体产生了最多6±2个和11±3个再生芽。在添加了激动素的培养基上连续培养外植体可支持离体开花。花朵较小且不结实。添加萘乙酸与细胞分裂素一起可支持愈伤组织形成。然而，单独使用萘乙酸对离体生根非常有利。发育成的植株被转移到土壤中。在珠孔端对面进行小切口后，种子发芽率得到提高。一个小切口有助于检查种子质量，还将种子发芽率从2%提高到了93%。赤霉素（2或5mg/l）不影响打破种子休眠。在胚根出现后，这些种子被转移到添加了诱导子（水杨酸或茉莉酸）的MS培养基上。从处理后的第6天到第18天记录幼苗的生长参数。尽管诱导子降低了幼苗的生长，但随着水杨酸/茉莉酸浓度的增加，抗坏血酸过氧化物酶活性提高了10 - 14倍。已知诱导子可增强植物中的次生代谢产物（补骨脂素），补骨脂素合酶基因三倍的表达上调也证明了这一点。该研究通过直接和间接器官发生成功建立了补骨脂的高效再生方案，显著提高了芽再生、种子发芽和离体开花。此外，诱导子处理不仅诱导了应激反应，还促进了更高的补骨脂素积累，使这种方法有望用于大规模繁殖和次生代谢产物生产。
