Ratajczak-Zacharko Weronika, Skonieczna-Żydecka Karolina, Laszczyńska Maria, Sipak Olimpia, Lubkowska Anna
Department of Functional Diagnostics and Physical Medicine, Faculty of Life Science, Pomeranian Medical University, Szczecin, 71-210, Poland.
Department of Biochemical Sciences, Faculty of Life Science, Pomeranian Medical University, Szczecin, 71‑460, Poland.
Sci Rep. 2025 May 4;15(1):15603. doi: 10.1038/s41598-025-00466-9.
The intestinal microbiota, in terms of both composition and functioning, exerts a significant influence on the human body. Disturbed microbiota is a common occurrence in the context of numerous diseases. The available evidence increasingly points to a correlation between this condition and the development of prostate diseases, including benign prostatic hyperplasia and prostate cancer. 16 S sequencing of the V3-V4 region was performed and then evaluated alpha and beta diversity of the faeces microbiota of healthy (control group, N = 81) and BPH patients (study group, N = 76). The exploration of enterotypes involved the application of the Dirichlet-Multinomial model, executed for selecting community types. The study revealed no statistically significant difference in alpha diversity between the control group and the group of patients diagnosed with BPH. However, a significant difference was observed in beta diversity (Permanova test: F-value = 5.56, p-value < 0.001). The identification of enterotypes revealed significant differences between the healthy male cohort and those diagnosed with BPH (p = 0.035). In the cohort of men with BPH, the most prevalent was enterotype 3, characterized by a predominance of Blautia, Bacteroides, and Streptococcus. The occurrence of enterotype 3 was associated with an increased likelihood of BPH, exceeding threefold that of enterotype 1 (OR = 3.24). These findings suggest that alterations in the gut microbiota, particularly the presence of enterotype 3, may serve as a microbiological pattern associated with BPH.
肠道微生物群在组成和功能方面对人体都有重大影响。微生物群紊乱在许多疾病的背景下很常见。现有证据越来越多地表明这种情况与前列腺疾病（包括良性前列腺增生和前列腺癌）的发生之间存在关联。对V3-V4区域进行了16S测序，然后评估了健康人（对照组，N = 81）和良性前列腺增生患者（研究组，N = 76）粪便微生物群的α和β多样性。肠道类型的探索涉及应用狄利克雷多项模型，用于选择群落类型。研究发现，对照组与被诊断为良性前列腺增生的患者组之间在α多样性方面没有统计学上的显著差异。然而，在β多样性方面观察到了显著差异（PERMANOVA检验：F值 = 5.56，p值 < 0.001）。肠道类型的鉴定显示，健康男性队列与被诊断为良性前列腺增生的男性队列之间存在显著差异（p = 0.035）。在患有良性前列腺增生的男性队列中，最常见的是肠道类型3，其特征是布劳特氏菌属、拟杆菌属和链球菌属占主导地位。肠道类型3的出现与良性前列腺增生的可能性增加有关，超过肠道类型1的三倍（OR = 3.24）。这些发现表明，肠道微生物群的改变，特别是肠道类型3的存在，可能是与良性前列腺增生相关的微生物学模式。
