Maino V C, Kurnick J T, Kubo R T, Grey H M
J Immunol. 1977 Mar;118(3):742-8.
The response of CLL (chronic lymphatic leukemia) lymphocytes to PHA, PWM, and Con A with respect to changes in surface markers and synthesis and secretion of immunoglobulin were examined. After PHA stimulation the percentage of cells bearing readily detectable surface immunoglobulin (SmIg) diminished rapidly whereas cells forming rosettes with sheep erythrocytes (E-rosettes) increased from less than 1% to 30 to 50%. The great majority of blast-transformed cells were E-rosette-positive cells with a small population of SmIg-positive blast cells also observed. Ig production in four of seven CLL lymphocyte populations was increased 2.5 to greater than 40-fold after 4 to 6 days of culture in the presence of PHA. In contrast, pokeweed mitogen did not affect Ig synthesis. Furthermore, the Ig secreted into the culture supernatant fluids from seven of eight CLL cases examined consisted almost entirely of free light chain molecules. In contrast, the cell lysates contained a significant proportion of intact Ig molecules. These results indicate that CLL cells can, under certain circumstances, be stimulated to synthesize and secrete increased amounts of Ig, but that there is a basic defect in the biosynthetic mechanism of these cells which result in the secretion of free light chains rather than intact immunoglobulin molecules.
研究了慢性淋巴细胞白血病（CLL）淋巴细胞对植物血凝素（PHA）、美洲商陆有丝分裂原（PWM）和刀豆蛋白A（Con A）的反应，涉及表面标志物的变化以及免疫球蛋白的合成和分泌。PHA刺激后，带有易于检测到的表面免疫球蛋白（SmIg）的细胞百分比迅速下降，而与绵羊红细胞形成玫瑰花结的细胞（E-玫瑰花结）从不到1%增加到30%至50%。绝大多数母细胞转化细胞是E-玫瑰花结阳性细胞，也观察到一小部分SmIg阳性母细胞。在PHA存在的情况下培养4至6天后，七个CLL淋巴细胞群体中的四个群体的免疫球蛋白产量增加了2.5至40倍以上。相比之下，商陆有丝分裂原不影响免疫球蛋白的合成。此外，在检测的八个CLL病例中的七个病例中，分泌到培养上清液中的免疫球蛋白几乎完全由游离轻链分子组成。相比之下，细胞裂解物中含有相当比例的完整免疫球蛋白分子。这些结果表明，CLL细胞在某些情况下可以被刺激合成和分泌增加量的免疫球蛋白，但这些细胞的生物合成机制存在基本缺陷，导致分泌游离轻链而不是完整的免疫球蛋白分子。
