单细胞老年血液-脑脊液图谱揭示正常压力脑积水中外周影响的免疫失调。

Single-cell elderly blood-CSF atlas implicates peripherally influenced immune dysregulation in normal pressure hydrocephalus.

作者信息

Duy Phan Q, Kiziltug Emre, Greenberg Ana B W, Mehta Neel H, Hao Le T, Fortes Carla, Mullany Susan, Fan Baojian, Manichaikul Ani, Teich Andrew F, Chan Diane, Alper Seth L, Hyman Bradley T, Arnold Steven E, McKhann Guy M, Frosch Matthew P, Kahle Kristopher T

机构信息

Department of Neurosurgery, Massachusetts General Hospital, Boston, MA 02114.

Department of Genome Sciences, Center for Public Health Genomics, University of Virginia, Charlottesville, VA 22903.

出版信息

Proc Natl Acad Sci U S A. 2025 May 13;122(19):e2412159122. doi: 10.1073/pnas.2412159122. Epub 2025 May 5.

DOI:10.1073/pnas.2412159122

PMID:40324076

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12087963/

Abstract

We have generated a single-cell RNA sequencing atlas of peripheral blood and ventricular CSF in idiopathic normal pressure hydrocephalus (iNPH) patients totaling 140,207 single-cell transcriptomes. We found proinflammatory alterations in peripheral blood and CSF monocytes in iNPH patients with lower baseline cognitive function. We also identified CSF cell populations likely representing periventricular sloughing of degenerating neuroglial cells. Our findings suggest possible immune dysregulation in the blood and CSF of iNPH patients.

摘要

我们生成了特发性正常压力脑积水（iNPH）患者外周血和脑室脑脊液的单细胞RNA测序图谱，共包含140,207个单细胞转录组。我们发现基线认知功能较低的iNPH患者外周血和脑脊液单核细胞存在促炎改变。我们还鉴定出可能代表脑室周围神经胶质细胞退变脱落的脑脊液细胞群。我们的研究结果表明iNPH患者血液和脑脊液中可能存在免疫失调。