Sheng Y B, Badger T M, Asplund J M, Wixom R L
J Nutr. 1977 Apr;107(4):621-30. doi: 10.1093/jn/107.4.621.
An adult male fed by parenteral alimentation for 48 days had successive intakes of a complete mixture of essential amino acids, and then lysine- and histidine deletion to test for the essentially of histidine. After 22 days of histidine-free-intake, 15NH4Cl was ingested orally with subsequent measurement of the time patterns for the appearance of 15N in total plasma proteins, globin from hemoglobin and urine. The 15N was incorporated into glutamate, aspartate, tyrosine, and histidine, but not lysine, of plasma proteins and globin. Urinary histidine and Ntau-methylhistidine, but not lysine, also contained 15N. Degradation of several histidine samples by two methods and two Ntau-methylhistidine samples demonstrated the presence of 15N in both the alpha-amino nitrogen and the imidazole ring. The incorporation of 15N into the ring indicates limited synthesis of histidine in an adult man under the above stated conditions.
一名成年男性接受了48天的肠外营养，先后摄入了必需氨基酸的完整混合物，然后进行赖氨酸和组氨酸缺失试验以检测组氨酸的必需性。在无组氨酸摄入22天后，口服15NH4Cl，随后测量15N在总血浆蛋白、血红蛋白中的珠蛋白和尿液中出现的时间模式。15N被掺入血浆蛋白和珠蛋白的谷氨酸、天冬氨酸、酪氨酸和组氨酸中，但未掺入赖氨酸中。尿中的组氨酸和Nτ-甲基组氨酸也含有15N，但赖氨酸不含。通过两种方法对几个组氨酸样品和两个Nτ-甲基组氨酸样品进行降解，结果表明α-氨基氮和咪唑环中均存在15N。15N掺入环中表明在上述条件下成年男性体内组氨酸的合成有限。
